1jf9: Difference between revisions
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[[Image:1jf9.gif|left|200px]] | [[Image:1jf9.gif|left|200px]] | ||
'''Crystal Structure of selenocysteine lyase''' | {{Structure | ||
|PDB= 1jf9 |SIZE=350|CAPTION= <scene name='initialview01'>1jf9</scene>, resolution 2.Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=PLP:PYRIDOXAL-5'-PHOSPHATE'>PLP</scene> | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/Selenocysteine_lyase Selenocysteine lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.4.1.16 4.4.1.16] | |||
|GENE= NIFS/CSDB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | |||
}} | |||
'''Crystal Structure of selenocysteine lyase''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1JF9 is a [ | 1JF9 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JF9 OCA]. | ||
==Reference== | ==Reference== | ||
Analysis of the E. coli NifS CsdB protein at 2.0 A reveals the structural basis for perselenide and persulfide intermediate formation., Lima CD, J Mol Biol. 2002 Feb 1;315(5):1199-208. PMID:[http:// | Analysis of the E. coli NifS CsdB protein at 2.0 A reveals the structural basis for perselenide and persulfide intermediate formation., Lima CD, J Mol Biol. 2002 Feb 1;315(5):1199-208. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11827487 11827487] | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Selenocysteine lyase]] | [[Category: Selenocysteine lyase]] | ||
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[[Category: cysteine]] | [[Category: cysteine]] | ||
[[Category: new york structural genomix research consortium]] | [[Category: new york structural genomix research consortium]] | ||
[[Category: | [[Category: nif]] | ||
[[Category: nysgxrc]] | [[Category: nysgxrc]] | ||
[[Category: protein structure initiative]] | [[Category: protein structure initiative]] | ||
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[[Category: pursulfide]] | [[Category: pursulfide]] | ||
[[Category: selenocysteine]] | [[Category: selenocysteine]] | ||
[[Category: structural | [[Category: structural genomic]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:02:40 2008'' | ||
Revision as of 13:02, 20 March 2008
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| , resolution 2.Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | NIFS/CSDB (Escherichia coli) | ||||||
| Activity: | Selenocysteine lyase, with EC number 4.4.1.16 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of selenocysteine lyase
OverviewOverview
The Escherichia coli NifS CsdB protein is a member of the homodimeric pyridoxal 5'-phosphate (PLP)-dependent family of enzymes. These enzymes are capable of decomposing cysteine or selenocysteine into L-alanine and sulfur or selenium, respectively. E. coli NifS CsdB has a high specificity for L-selenocysteine in comparison to l-cysteine, suggesting a role for this enzyme is selenium metabolism. The 2.0 A crystal structure of E. coli NifS CsdB reveals a high-resolution view of the active site of this enzyme in apo-, persulfide, perselenide, and selenocysteine-bound intermediates, suggesting a mechanism for the stabilization of the enzyme persulfide and perselenide intermediates during catalysis, a necessary intermediate in the formation of sulfur and selenium containing metabolites.
About this StructureAbout this Structure
1JF9 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
ReferenceReference
Analysis of the E. coli NifS CsdB protein at 2.0 A reveals the structural basis for perselenide and persulfide intermediate formation., Lima CD, J Mol Biol. 2002 Feb 1;315(5):1199-208. PMID:11827487
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