1j4s: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:1j4s.jpg|left|200px]] | [[Image:1j4s.jpg|left|200px]] | ||
'''Structure of Artocarpin: a Lectin with Mannose Specificity (Form 1)''' | {{Structure | ||
|PDB= 1j4s |SIZE=350|CAPTION= <scene name='initialview01'>1j4s</scene>, resolution 2.5Å | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''Structure of Artocarpin: a Lectin with Mannose Specificity (Form 1)''' | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
1J4S is a [ | 1J4S is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Artocarpus_integer Artocarpus integer]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J4S OCA]. | ||
==Reference== | ==Reference== | ||
Crystal structures of artocarpin, a Moraceae lectin with mannose specificity, and its complex with methyl-alpha-D-mannose: implications to the generation of carbohydrate specificity., Pratap JV, Jeyaprakash AA, Rani PG, Sekar K, Surolia A, Vijayan M, J Mol Biol. 2002 Mar 22;317(2):237-47. PMID:[http:// | Crystal structures of artocarpin, a Moraceae lectin with mannose specificity, and its complex with methyl-alpha-D-mannose: implications to the generation of carbohydrate specificity., Pratap JV, Jeyaprakash AA, Rani PG, Sekar K, Surolia A, Vijayan M, J Mol Biol. 2002 Mar 22;317(2):237-47. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11902840 11902840] | ||
[[Category: Artocarpus integer]] | [[Category: Artocarpus integer]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 23: | Line 32: | ||
[[Category: greek key motif]] | [[Category: greek key motif]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:58:51 2008'' | ||
Revision as of 12:58, 20 March 2008
| |||||||
| , resolution 2.5Å | |||||||
|---|---|---|---|---|---|---|---|
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structure of Artocarpin: a Lectin with Mannose Specificity (Form 1)
OverviewOverview
The seeds of jack fruit (Artocarpus integrifolia) contain two tetrameric lectins, jacalin and artocarpin. Jacalin was the first lectin found to exhibit the beta-prism I fold, which is characteristic of the Moraceae plant lectin family. Jacalin contains two polypeptide chains produced by a post-translational proteolysis which has been shown to be crucial for generating its specificity for galactose. Artocarpin is a single chain protein with considerable sequence similarity with jacalin. It, however, exhibits many properties different from those of jacalin. In particular, it is specific to mannose. The structures of two crystal forms, form I and form II, of the native lectin have been determined at 2.4 and 2.5 A resolution, respectively. The structure of the lectin complexed with methyl-alpha-mannose, has also been determined at 2.9 A resolution. The structure is similar to jacalin, although differences exist in details. The crystal structures and detailed modelling studies indicate that the following differences between the carbohydrate binding sites of artocarpin and jacalin are responsible for the difference in the specificities of the two lectins. Firstly, artocarpin does not contain, unlike jacalin, an N terminus generated by post-translational proteolysis. Secondly, there is no aromatic residue in the binding site of artocarpin whereas there are four in that of jacalin. A comparison with similar lectins of known structures or sequences, suggests that, in general, stacking interactions with aromatic residues are important for the binding of galactose while such interactions are usually absent in the carbohydrate binding sites of mannose-specific lectins with the beta-prism I fold.
About this StructureAbout this Structure
1J4S is a Single protein structure of sequence from Artocarpus integer. Full crystallographic information is available from OCA.
ReferenceReference
Crystal structures of artocarpin, a Moraceae lectin with mannose specificity, and its complex with methyl-alpha-D-mannose: implications to the generation of carbohydrate specificity., Pratap JV, Jeyaprakash AA, Rani PG, Sekar K, Surolia A, Vijayan M, J Mol Biol. 2002 Mar 22;317(2):237-47. PMID:11902840
Page seeded by OCA on Thu Mar 20 11:58:51 2008