1gt1: Difference between revisions

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-{{STRUCTURE_1gt1|  PDB=1gt1  |  SCENE=  }}
+==Complex of Bovine Odorant Binding Protein with Aminoanthracene and pyrazine==
-===Complex of Bovine Odorant Binding Protein with Aminoanthracene and pyrazine===
+<StructureSection load='1gt1' size='340' side='right' caption='[[1gt1]], [[Resolution|resolution]] 1.71&Aring;' scene=''>
-{{ABSTRACT_PUBMED_15373829}}
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1gt1]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GT1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1GT1 FirstGlance]. <br>
+</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=3OM:(3S)-1-OCTEN-3-OL'>3OM</scene>, <scene name='pdbligand=ANC:ANTHRACEN-1-YLAMINE'>ANC</scene>, <scene name='pdbligand=PRZ:2-ISOBUTYL-3-METHOXYPYRAZINE'>PRZ</scene><br>
+<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1g85|1g85]], [[1gt3|1gt3]], [[1gt4|1gt4]], [[1gt5|1gt5]], [[1hn2|1hn2]], [[1obp|1obp]], [[1pbo|1pbo]]</td></tr>
+<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gt1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gt1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1gt1 RCSB], [http://www.ebi.ac.uk/pdbsum/1gt1 PDBsum]</span></td></tr>
+<table>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gt/1gt1_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The structure of bovine odorant-binding protein (bOBP) revealed a striking feature of a dimer formed by domain swapping [Tegoni, M., Ramoni, R., Bignetti, E., Spinelli, S. &amp; Cambillau, C. (1996) Nat. Struct. Biol.3, 863-867; Bianchet, M.A., Bains, G., Pelosi, P., Pevsner, J., Snyder, S.H., Monaco, H.L. &amp; Amzel, L.M. (1996) Nat. Struct. Biol.3, 934-939] and the presence of a naturally occuring ligand [Ramoni, R., Vincent, F., Grolli, S., Conti, V., Malosse, C., Boyer, F.D., Nagnan-Le Meillour, P., Spinelli, S., Cambillau, C. &amp; Tegoni, M. (2001) J. Biol. Chem.276, 7150-7155]. These features led us to investigate the binding of odorant molecules with bOBP in solution and in the crystal. The behavior of odorant molecules in bOBP resembles that observed with porcine OBP (pOBP), although the latter is monomeric and devoid of ligand when purified. The odorant molecules presented K(d) values with bOBP in the micromolar range. Most of the X-ray structures revealed that odorant molecules interact with a common set of residues forming the cavity wall and do not exhibit specific interactions. Depending on the ligand and on the monomer (A or B), a single residue--Phe89--presents alternate conformations and might control cross-talking between the subunits. Crystal data on both pOBP and bOBP, in contrast with binding and spectroscopic studies on rat OBP in solution, reveal an absence of significant conformational changes involving protein loops or backbone. Thus, the role of OBP in signal triggering remains unresolved.
-==Function==
+Crystal structures of bovine odorant-binding protein in complex with odorant molecules.,Vincent F, Ramoni R, Spinelli S, Grolli S, Tegoni M, Cambillau C Eur J Biochem. 2004 Oct;271(19):3832-42. PMID:15373829<ref>PMID:15373829</ref>
-[[http://www.uniprot.org/uniprot/OBP_BOVIN OBP_BOVIN]] This protein binds a wide variety of chemical odorants.
-==About this Structure==
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[1gt1]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GT1 OCA].
+</div>
 ==See Also==
 *[[Odorant binding protein|Odorant binding protein]]
+== References ==
-==Reference==
+<references/>
-<ref group="xtra">PMID:015373829</ref><references group="xtra"/><references/>
+__TOC__
+</StructureSection>
 [[Category: Bos taurus]]
 [[Category: Cambillau, C.]]