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{{STRUCTURE_3a3y| PDB=3a3y | SCENE= }}
==Crystal structure of the sodium-potassium pump with bound potassium and ouabain==
===Crystal structure of the sodium-potassium pump with bound potassium and ouabain===
<StructureSection load='3a3y' size='340' side='right' caption='[[3a3y]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
{{ABSTRACT_PUBMED_19666591}}
== Structural highlights ==
<table><tr><td colspan='2'>[[3a3y]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Squalus_acanthias Squalus acanthias]. The October 2009 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Sodium-Potassium Pump'' by David Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2009_10 10.2210/rcsb_pdb/mom_2009_10]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3A3Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3A3Y FirstGlance]. <br>
</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CLR:CHOLESTEROL'>CLR</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MF4:TETRAFLUOROMAGNESATE(2-)'>MF4</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=OBN:OUABAIN'>OBN</scene><br>
<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2zxe|2zxe]]</td></tr>
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3a3y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3a3y OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3a3y RCSB], [http://www.ebi.ac.uk/pdbsum/3a3y PDBsum]</span></td></tr>
<table>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a3/3a3y_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The sodium-potassium pump (Na(+),K(+)-ATPase) is responsible for establishing Na(+) and K(+) concentration gradients across the plasma membrane and therefore plays an essential role in, for instance, generating action potentials. Cardiac glycosides, prescribed for congestive heart failure for more than 2 centuries, are efficient inhibitors of this ATPase. Here we describe a crystal structure of Na(+),K(+)-ATPase with bound ouabain, a representative cardiac glycoside, at 2.8 A resolution in a state analogous to E2.2K(+).Pi. Ouabain is deeply inserted into the transmembrane domain with the lactone ring very close to the bound K(+), in marked contrast to previous models. Due to antagonism between ouabain and K(+), the structure represents a low-affinity ouabain-bound state. Yet, most of the mutagenesis data obtained with the high-affinity state are readily explained by the present crystal structure, indicating that the binding site for ouabain is essentially the same. According to a homology model for the high affinity state, it is a closure of the binding cavity that confers a high affinity.


==About this Structure==
Crystal structure of the sodium-potassium pump (Na+,K+-ATPase) with bound potassium and ouabain.,Ogawa H, Shinoda T, Cornelius F, Toyoshima C Proc Natl Acad Sci U S A. 2009 Aug 18;106(33):13742-7. Epub 2009 Aug 3. PMID:19666591<ref>PMID:19666591</ref>
[[3a3y]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Squalus_acanthias Squalus acanthias]. The October 2009 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Sodium-Potassium Pump''  by David Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2009_10 10.2210/rcsb_pdb/mom_2009_10]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3A3Y OCA].
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>


==See Also==
==See Also==
*[[ATPase|ATPase]]
*[[ATPase|ATPase]]
*[[Sodium-Potassium ATPase|Sodium-Potassium ATPase]]
== References ==
 
<references/>
==Reference==
__TOC__
<ref group="xtra">PMID:019666591</ref><references group="xtra"/>
</StructureSection>
[[Category: RCSB PDB Molecule of the Month]]
[[Category: RCSB PDB Molecule of the Month]]
[[Category: Sodium-Potassium Pump]]
[[Category: Sodium-Potassium Pump]]

Revision as of 08:00, 2 October 2014

Crystal structure of the sodium-potassium pump with bound potassium and ouabainCrystal structure of the sodium-potassium pump with bound potassium and ouabain

Structural highlights

3a3y is a 3 chain structure with sequence from Squalus acanthias. The October 2009 RCSB PDB Molecule of the Month feature on Sodium-Potassium Pump by David Goodsell is 10.2210/rcsb_pdb/mom_2009_10. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:, , , , ,
Related:2zxe
Resources:FirstGlance, OCA, RCSB, PDBsum

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The sodium-potassium pump (Na(+),K(+)-ATPase) is responsible for establishing Na(+) and K(+) concentration gradients across the plasma membrane and therefore plays an essential role in, for instance, generating action potentials. Cardiac glycosides, prescribed for congestive heart failure for more than 2 centuries, are efficient inhibitors of this ATPase. Here we describe a crystal structure of Na(+),K(+)-ATPase with bound ouabain, a representative cardiac glycoside, at 2.8 A resolution in a state analogous to E2.2K(+).Pi. Ouabain is deeply inserted into the transmembrane domain with the lactone ring very close to the bound K(+), in marked contrast to previous models. Due to antagonism between ouabain and K(+), the structure represents a low-affinity ouabain-bound state. Yet, most of the mutagenesis data obtained with the high-affinity state are readily explained by the present crystal structure, indicating that the binding site for ouabain is essentially the same. According to a homology model for the high affinity state, it is a closure of the binding cavity that confers a high affinity.

Crystal structure of the sodium-potassium pump (Na+,K+-ATPase) with bound potassium and ouabain.,Ogawa H, Shinoda T, Cornelius F, Toyoshima C Proc Natl Acad Sci U S A. 2009 Aug 18;106(33):13742-7. Epub 2009 Aug 3. PMID:19666591[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

See Also

References

  1. ↑ Ogawa H, Shinoda T, Cornelius F, Toyoshima C. Crystal structure of the sodium-potassium pump (Na+,K+-ATPase) with bound potassium and ouabain. Proc Natl Acad Sci U S A. 2009 Aug 18;106(33):13742-7. Epub 2009 Aug 3. PMID:19666591

3a3y, resolution 2.80Å

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