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[[Image: | ==STRUCTURE OF THE 70S RIBOSOME BOUND TO RELEASE FACTOR 2 AND A SUBSTRATE ANALOG PROVIDES INSIGHTS INTO CATALYSIS OF PEPTIDE RELEASE== | ||
<StructureSection load='2x9r' size='340' side='right' caption='[[2x9r]], [[Resolution|resolution]] 3.10Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[2x9r]] is a 25 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X9R OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2X9R FirstGlance]. <br> | |||
</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br> | |||
<tr><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=8AN:3-AMINO-3-DEOXYADENOSINE+5-(DIHYDROGEN+PHOSPHATE)'>8AN</scene>, <scene name='pdbligand=PHA:PHENYLALANINAL'>PHA</scene></td></tr> | |||
<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2x9s|2x9s]], [[2x9t|2x9t]], [[2x9u|2x9u]], [[1ibk|1ibk]], [[1ibl|1ibl]], [[2uuc|2uuc]], [[2wdk|2wdk]], [[2j02|2j02]], [[1j5e|1j5e]], [[2wrq|2wrq]], [[2j00|2j00]], [[1hnx|1hnx]], [[2b9o|2b9o]], [[2wh3|2wh3]], [[2uub|2uub]], [[1vov|1vov]], [[1emi|1emi]], [[1pnx|1pnx]], [[2v46|2v46]], [[2vqf|2vqf]], [[2wh1|2wh1]], [[1n32|1n32]], [[2wrn|2wrn]], [[2f4v|2f4v]], [[1twt|1twt]], [[1qzc|1qzc]], [[2uxd|2uxd]], [[1fka|1fka]], [[1fjg|1fjg]], [[1n36|1n36]], [[2uxc|2uxc]], [[1hnw|1hnw]], [[1l1u|1l1u]], [[1hr0|1hr0]], [[2v48|2v48]], [[2b64|2b64]], [[1xmq|1xmq]], [[2b9m|2b9m]], [[1pn8|1pn8]], [[2wdg|2wdg]], [[1xnr|1xnr]], [[1hnz|1hnz]], [[1dv4|1dv4]], [[1rss|1rss]], [[2wdh|2wdh]], [[1jgq|1jgq]], [[1i94|1i94]], [[1i96|1i96]], [[2uu9|2uu9]], [[1pn7|1pn7]], [[1eg0|1eg0]], [[1ibm|1ibm]], [[2uxb|2uxb]], [[2vqe|2vqe]], [[1g1x|1g1x]], [[1i95|1i95]], [[2uua|2uua]], [[1xnq|1xnq]], [[1qd7|1qd7]], [[1gix|1gix]], [[1jgo|1jgo]], [[1jgp|1jgp]], [[1n34|1n34]], [[1n33|1n33]], [[1pns|1pns]], [[1yl4|1yl4]], [[2wdm|2wdm]], [[1xmo|1xmo]], [[1i97|1i97]]</td></tr> | |||
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2x9r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2x9r OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2x9r RCSB], [http://www.ebi.ac.uk/pdbsum/2x9r PDBsum]</span></td></tr> | |||
<table> | |||
== Evolutionary Conservation == | |||
[[Image:Consurf_key_small.gif|200px|right]] | |||
Check<jmol> | |||
<jmolCheckbox> | |||
<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/x9/2x9r_consurf.spt"</scriptWhenChecked> | |||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | |||
<text>to colour the structure by Evolutionary Conservation</text> | |||
</jmolCheckbox> | |||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf]. | |||
<div style="clear:both"></div> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
We report the crystal structure of release factor 2 bound to ribosome with an aminoacyl tRNA substrate analog at the ribosomal P site, at 3.1 A resolution. The structure shows that upon stop-codon recognition, the universally conserved GGQ motif packs tightly into the peptidyl transferase center. Nucleotide A2602 of 23S rRNA, implicated in peptide release, packs with the GGQ motif in release factor 2. The ribose of A76 of the peptidyl-tRNA adopts the C2'-endo conformation, and the 2' hydroxyl of A76 is within hydrogen-bond distance of the 2' hydroxyl of A2451. The structure suggests how a catalytic water can be coordinated in the peptidyl transferase center and, together with previous biochemical and computational data, suggests a model for how the ester bond between the peptidyl tRNA and the nascent peptide is hydrolyzed. | |||
Structure of the 70S ribosome bound to release factor 2 and a substrate analog provides insights into catalysis of peptide release.,Jin H, Kelley AC, Loakes D, Ramakrishnan V Proc Natl Acad Sci U S A. 2010 Apr 26. PMID:20421507<ref>PMID:20421507</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
==See Also== | ==See Also== | ||
*[[Ribosomal protein THX|Ribosomal protein THX]] | *[[Ribosomal protein THX|Ribosomal protein THX]] | ||
*[[Ribosome 3D structures|Ribosome 3D structures]] | |||
== | == References == | ||
< | <references/> | ||
__TOC__ | |||
</StructureSection> | |||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Thermus thermophilus]] | [[Category: Thermus thermophilus]] | ||