2xgk: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[ | ==VIRUS LIKE PARTICLE OF L172W MUTANT OF MINUTE VIRUS OF MICE - THE IMMUNOSUPPRESSIVE STRAIN== | ||
<StructureSection load='2xgk' size='340' side='right' caption='[[2xgk]], [[Resolution|resolution]] 4.20Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[2xgk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Minute_virus_of_mice Minute virus of mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XGK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XGK FirstGlance]. <br> | |||
</td></tr><tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1mvm|1mvm]], [[1z1c|1z1c]]</td></tr> | |||
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xgk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xgk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2xgk RCSB], [http://www.ebi.ac.uk/pdbsum/2xgk PDBsum]</span></td></tr> | |||
<table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Parvovirus Minute Virus of Mice (MVM) packages a single copy of its linear single-stranded DNA genome into pre-formed capsids, in a process that is probably driven by a virus-encoded helicase. Parvoviruses have a roughly cylindrically shaped pore that surrounds each of the twelve fivefold vertices. The pore, which penetrates the virion shell, is created by the juxtaposition of ten anti-parallel beta-strands, two from each of the fivefold related capsid proteins. There is a bottleneck in the channel formed by the symmetry-related sidechains of the leucines at position 172. We report here the X-ray crystal structure of the particles produced by a leucine to tryptophan mutation at position 172 and the analysis of its biochemical properties. The mutant capsid has its fivefold channel blocked and the particles were unable to package DNA, strongly suggesting that the fivefold pore is the packaging portal for genome entry. | |||
Structure of a packaging defective mutant of Minute Virus of Mice indicates that the genome is packaged via a pore at a fivefold axis.,Plevka P, Hafenstein S, Li L, D'Abramo A Jr, Cotmore SF, Rossmann MG, Tattersall P J Virol. 2011 Mar 2. PMID:21367911<ref>PMID:21367911</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
==See Also== | ==See Also== | ||
*[[Virus coat protein|Virus coat protein]] | *[[Virus coat protein|Virus coat protein]] | ||
== References == | |||
== | <references/> | ||
< | __TOC__ | ||
</StructureSection> | |||
[[Category: Minute virus of mice]] | [[Category: Minute virus of mice]] | ||
[[Category: Abramo, A D.]] | [[Category: Abramo, A D.]] | ||
Revision as of 03:58, 2 October 2014
VIRUS LIKE PARTICLE OF L172W MUTANT OF MINUTE VIRUS OF MICE - THE IMMUNOSUPPRESSIVE STRAINVIRUS LIKE PARTICLE OF L172W MUTANT OF MINUTE VIRUS OF MICE - THE IMMUNOSUPPRESSIVE STRAIN
Structural highlights
Publication Abstract from PubMedParvovirus Minute Virus of Mice (MVM) packages a single copy of its linear single-stranded DNA genome into pre-formed capsids, in a process that is probably driven by a virus-encoded helicase. Parvoviruses have a roughly cylindrically shaped pore that surrounds each of the twelve fivefold vertices. The pore, which penetrates the virion shell, is created by the juxtaposition of ten anti-parallel beta-strands, two from each of the fivefold related capsid proteins. There is a bottleneck in the channel formed by the symmetry-related sidechains of the leucines at position 172. We report here the X-ray crystal structure of the particles produced by a leucine to tryptophan mutation at position 172 and the analysis of its biochemical properties. The mutant capsid has its fivefold channel blocked and the particles were unable to package DNA, strongly suggesting that the fivefold pore is the packaging portal for genome entry. Structure of a packaging defective mutant of Minute Virus of Mice indicates that the genome is packaged via a pore at a fivefold axis.,Plevka P, Hafenstein S, Li L, D'Abramo A Jr, Cotmore SF, Rossmann MG, Tattersall P J Virol. 2011 Mar 2. PMID:21367911[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
|
| ||||||||||||||||