4u8h: Difference between revisions

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-'''Unreleased structure'''
+==Crystal Structure of Mammalian Period-Cryptochrome Complex==
+<StructureSection load='4u8h' size='340' side='right' caption='[[4u8h]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[4u8h]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4U8H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4U8H FirstGlance]. <br>
+</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>
+<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4u8h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4u8h OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4u8h RCSB], [http://www.ebi.ac.uk/pdbsum/4u8h PDBsum]</span></td></tr>
+<table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The mammalian circadian clock is driven by a transcriptional-translational feedback loop, which produces robust 24-hr rhythms. Proper oscillation of the clock depends on the complex formation and periodic turnover of the Period and Cryptochrome proteins, which together inhibit their own transcriptional activator complex, CLOCK-BMAL1. We determined the crystal structure of the CRY-binding domain (CBD) of PER2 in complex with CRY2 at 2.8 A resolution. PER2-CBD adopts a highly extended conformation, embracing CRY2 with a sinuous binding mode. Its N-terminal end tucks into CRY adjacent to a large pocket critical for CLOCK-BMAL1 binding, while its C-terminal half flanks the CRY2 C-terminal helix and sterically hinders the recognition of CRY2 by the FBXL3 ubiquitin ligase. Unexpectedly, a strictly conserved intermolecular zinc finger, whose integrity is important for clock rhythmicity, further stabilizes the complex. Our structure-guided analyses show that these interspersed CRY-interacting regions represent multiple functional modules of PERs at the CRY-binding interface.DOI: http://dx.doi.org/10.7554/eLife.03674.001.
-The entry 4u8h is ON HOLD
+Molecular assembly of the period-cryptochrome circadian transcriptional repressor complex.,Nangle SN, Rosensweig C, Koike N, Tei H, Takahashi JS, Green CB, Zheng N Elife. 2014 Aug 15;3:e03674. doi: 10.7554/eLife.03674. PMID:25127877<ref>PMID:25127877</ref>
-Authors: Nangle, S.N., Rosensweig, C., Koike, N., Tei, H., Takahashi, J.S., Green, C.B., Zheng, N.
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-Description: Crystal Structure of Mammalian Period-Cryptochrome Complex
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Green, C B.]]
+[[Category: Koike, N.]]
+[[Category: Nangle, S N.]]
+[[Category: Rosensweig, C.]]
+[[Category: Takahashi, J S.]]
+[[Category: Tei, H.]]
+[[Category: Zheng, N.]]
+[[Category: Circadian clock protein-transcription complex]]
+[[Category: Transcriptional repression]]
+[[Category: Zinc-binding]]