1ik9: Difference between revisions
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[[Image:1ik9.jpg|left|200px]] | [[Image:1ik9.jpg|left|200px]] | ||
'''CRYSTAL STRUCTURE OF A XRCC4-DNA LIGASE IV COMPLEX''' | {{Structure | ||
|PDB= 1ik9 |SIZE=350|CAPTION= <scene name='initialview01'>1ik9</scene>, resolution 2.30Å | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/DNA_ligase_(ATP) DNA ligase (ATP)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.5.1.1 6.5.1.1] | |||
|GENE= XRCC4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), LIG4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | |||
}} | |||
'''CRYSTAL STRUCTURE OF A XRCC4-DNA LIGASE IV COMPLEX''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1IK9 is a [ | 1IK9 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. The following page contains interesting information on the relation of 1IK9 with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb55_1.html DNA Ligase]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IK9 OCA]. | ||
==Reference== | ==Reference== | ||
Crystal structure of an Xrcc4-DNA ligase IV complex., Sibanda BL, Critchlow SE, Begun J, Pei XY, Jackson SP, Blundell TL, Pellegrini L, Nat Struct Biol. 2001 Dec;8(12):1015-9. PMID:[http:// | Crystal structure of an Xrcc4-DNA ligase IV complex., Sibanda BL, Critchlow SE, Begun J, Pei XY, Jackson SP, Blundell TL, Pellegrini L, Nat Struct Biol. 2001 Dec;8(12):1015-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11702069 11702069] | ||
[[Category: DNA Ligase]] | [[Category: DNA Ligase]] | ||
[[Category: DNA ligase (ATP)]] | [[Category: DNA ligase (ATP)]] | ||
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[[Category: v(d)j recombination]] | [[Category: v(d)j recombination]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:51:27 2008'' | ||
Revision as of 12:51, 20 March 2008
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| , resolution 2.30Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | XRCC4 (Homo sapiens), LIG4 (Homo sapiens) | ||||||
| Activity: | DNA ligase (ATP), with EC number 6.5.1.1 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF A XRCC4-DNA LIGASE IV COMPLEX
OverviewOverview
A complex of two proteins, Xrcc4 and DNA ligase IV, plays a fundamental role in DNA non-homologous end joining (NHEJ), a cellular function required for double-strand break repair and V(D)J recombination. Here we report the crystal structure of human Xrcc4 bound to a polypeptide that corresponds to the DNA ligase IV sequence linking its two BRCA1 C-terminal (BRCT) domains. In the complex, a single ligase chain binds asymmetrically to an Xrcc4 dimer. The helical tails of Xrcc4 undergo a substantial conformational change relative to the uncomplexed protein, forming a coiled coil that unwinds upon ligase binding, leading to a flat interaction surface. A buried network of charged hydrogen bonds surrounded by extensive hydrophobic contacts explains the observed tightness of the interaction. The strong conservation of residues at the interface between the two proteins provides evidence that the observed mode of interaction has been maintained in NHEJ throughout evolution.
DiseaseDisease
Known diseases associated with this structure: LIG4 syndrome OMIM:[601837], Multiple myeloma, resistance to OMIM:[601837]
About this StructureAbout this Structure
1IK9 is a Protein complex structure of sequences from Homo sapiens. The following page contains interesting information on the relation of 1IK9 with [DNA Ligase]. Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of an Xrcc4-DNA ligase IV complex., Sibanda BL, Critchlow SE, Begun J, Pei XY, Jackson SP, Blundell TL, Pellegrini L, Nat Struct Biol. 2001 Dec;8(12):1015-9. PMID:11702069
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