1ij0: Difference between revisions

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Revision as of 12:50, 20 March 2008

File:1ij0.jpg

, resolution 1.86Å

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Coiled Coil Trimer GCN4-pVLS Ser at Buried D Position

OverviewOverview

Coiled coils, estimated to constitute 3-5% of the encoded residues in most genomes, are characterized by a heptad repeat, (abcdefg)(n), where the buried a and d positions form the interface between multiple alpha-helices. Although generally hydrophobic, a substantial fraction ( approximately 20%) of these a- and d-position residues are polar or charged. We constructed variants of the well-characterized coiled coil GCN4-p1 with a single polar residue (Asn, Gln, Ser, or Thr) at either an a or a d position. The stability and oligomeric specificity of each variant were measured, and crystal structures of coiled-coil trimers with threonine or serine at either an a or a d position were determined. The structures show how single polar residues in the interface affect not only local packing, but also overall coiled-coil geometry as seen by changes in the Crick supercoil parameters and core cavity volumes.

About this StructureAbout this Structure

1IJ0 is a Single protein structure of sequence from [1]. Full crystallographic information is available from OCA.

ReferenceReference

Buried polar residues in coiled-coil interfaces., Akey DL, Malashkevich VN, Kim PS, Biochemistry. 2001 May 29;40(21):6352-60. PMID:11371197

Page seeded by OCA on Thu Mar 20 11:50:59 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1ij0.jpg|left|200px]]<br /><applet load="1ij0" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1ij0.jpg|left|200px]]
-caption="1ij0, resolution 1.86&Aring;" />
-'''Coiled Coil Trimer GCN4-pVLS Ser at Buried D Position'''<br />
+ {{Structure
+|PDB= 1ij0 |SIZE=350|CAPTION= <scene name='initialview01'>1ij0</scene>, resolution 1.86&Aring;
+|SITE=
+|LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> and <scene name='pdbligand=ACE:ACETYL GROUP'>ACE</scene>
+|ACTIVITY=
+|GENE=
+}}
+'''Coiled Coil Trimer GCN4-pVLS Ser at Buried D Position'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-IJ0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=ZN:'>ZN</scene> and <scene name='pdbligand=ACE:'>ACE</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IJ0 OCA].
+IJ0 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IJ0 OCA].
 ==Reference==
-Buried polar residues in coiled-coil interfaces., Akey DL, Malashkevich VN, Kim PS, Biochemistry. 2001 May 29;40(21):6352-60. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11371197 11371197]
+Buried polar residues in coiled-coil interfaces., Akey DL, Malashkevich VN, Kim PS, Biochemistry. 2001 May 29;40(21):6352-60. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11371197 11371197]
 [[Category: Single protein]]
 [[Category: Akey, D L.]]
@@ Line 19: / Line 28: @@
 [[Category: coiled coil trimer]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:12:22 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:50:59 2008''