1if1: Difference between revisions

Revision as of 12:49, 20 March 2008

, resolution 3.000Å

Coordinates:

INTERFERON REGULATORY FACTOR 1 (IRF-1) COMPLEX WITH DNA

OverviewOverview

The family of interferon regulatory factor (IRF) transcription factors is important in the regulation of interferons in response to infection by virus and in the regulation of interferon-inducible genes. The IRF family is characterized by a unique 'tryptophan cluster' DNA-binding region. Here we report the crystal structure of the IRF-1 region bound to the natural positive regulatory domain I (PRD I) DNA element from the interferon-beta promoter. The structure provides the first three-dimensional view of a member of the growing IRF family, revealing a new helix-turn-helix motif that latches onto DNA through three of the five conserved tryptophans. The motif selects a short GAAA core sequence through an obliquely angled recognition helix, with an accompanying bending of the DNA axis in the direction of the protein. Together, these features suggest a basis for the occurrence of GAAA repeats within IRF response elements and provide clues to the assembly of the higher-order interferon-beta enhancesome.

About this StructureAbout this Structure

1IF1 is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.

ReferenceReference

Structure of IRF-1 with bound DNA reveals determinants of interferon regulation., Escalante CR, Yie J, Thanos D, Aggarwal AK, Nature. 1998 Jan 1;391(6662):103-6. PMID:9422515

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OCA

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-[[Image:1if1.gif|left|200px]]<br /><applet load="1if1" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1if1.gif|left|200px]]
-caption="1if1, resolution 3.000&Aring;" />
-'''INTERFERON REGULATORY FACTOR 1 (IRF-1) COMPLEX WITH DNA'''<br />
+ {{Structure
+|PDB= 1if1 |SIZE=350|CAPTION= <scene name='initialview01'>1if1</scene>, resolution 3.000&Aring;
+|SITE=
+|LIGAND=
+|ACTIVITY=
+|GENE=
+}}
+'''INTERFERON REGULATORY FACTOR 1 (IRF-1) COMPLEX WITH DNA'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-IF1 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IF1 OCA].
+IF1 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IF1 OCA].
 ==Reference==
-Structure of IRF-1 with bound DNA reveals determinants of interferon regulation., Escalante CR, Yie J, Thanos D, Aggarwal AK, Nature. 1998 Jan 1;391(6662):103-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9422515 9422515]
+Structure of IRF-1 with bound DNA reveals determinants of interferon regulation., Escalante CR, Yie J, Thanos D, Aggarwal AK, Nature. 1998 Jan 1;391(6662):103-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9422515 9422515]
 [[Category: Mus musculus]]
 [[Category: Single protein]]
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 [[Category: transcription factor]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:11:13 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:49:31 2008''