1idz: Difference between revisions

Revision as of 12:49, 20 March 2008

Coordinates:

STRUCTURE OF MYB TRANSFORMING PROTEIN, NMR, 20 STRUCTURES

OverviewOverview

A small globular protein, the third repeat of the c-Myb DNA-binding domain, which is composed of 54 amino acid residues, was engineered so as to understand the structural uniqueness of native proteins. This small protein has three alpha-helices that form a helix-turn-helix structure, which is maintained by the hydrophobic core with three Ile residues. One of the mutant proteins, with two of the buried Ile (Ile-155 and Ile-181) substituted with Leu residues, showed multiple conformations, as monitored by heteronuclear magnetic resonance spectroscopy for 13C- and 15N-labeled proteins. The increase in the side-chain conformational entropy, caused by changing the Ile to a Leu residue on an alpha-helix, could engender the lack of structural uniqueness. In native proteins, the conformations of not only the beta-branched side chains, but also those of the neighboring bulky side chains, can be greatly restricted, depending upon the local backbone structure.

About this StructureAbout this Structure

1IDZ is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.

ReferenceReference

A small engineered protein lacks structural uniqueness by increasing the side-chain conformational entropy., Furukawa K, Oda M, Nakamura H, Proc Natl Acad Sci U S A. 1996 Nov 26;93(24):13583-8. PMID:8942977

Page seeded by OCA on Thu Mar 20 11:49:06 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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-[[Image:1idz.jpg|left|200px]]<br /><applet load="1idz" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1idz.jpg|left|200px]]
-caption="1idz" />
-'''STRUCTURE OF MYB TRANSFORMING PROTEIN, NMR, 20 STRUCTURES'''<br />
+ {{Structure
+|PDB= 1idz |SIZE=350|CAPTION= <scene name='initialview01'>1idz</scene>
+|SITE=
+|LIGAND=
+|ACTIVITY=
+|GENE=
+}}
+'''STRUCTURE OF MYB TRANSFORMING PROTEIN, NMR, 20 STRUCTURES'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-IDZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IDZ OCA].
+IDZ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IDZ OCA].
 ==Reference==
-A small engineered protein lacks structural uniqueness by increasing the side-chain conformational entropy., Furukawa K, Oda M, Nakamura H, Proc Natl Acad Sci U S A. 1996 Nov 26;93(24):13583-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8942977 8942977]
+A small engineered protein lacks structural uniqueness by increasing the side-chain conformational entropy., Furukawa K, Oda M, Nakamura H, Proc Natl Acad Sci U S A. 1996 Nov 26;93(24):13583-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8942977 8942977]
 [[Category: Mus musculus]]
 [[Category: Single protein]]
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 [[Category: protooncogene product]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:10:52 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:49:06 2008''