1ia8: Difference between revisions

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Revision as of 12:47, 20 March 2008

File:1ia8.gif

, resolution 1.7Å

Ligands:

Coordinates:

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THE 1.7 A CRYSTAL STRUCTURE OF HUMAN CELL CYCLE CHECKPOINT KINASE CHK1

OverviewOverview

The checkpoint kinase Chk1 is an important mediator of cell cycle arrest following DNA damage. The 1.7 A resolution crystal structures of the human Chk1 kinase domain and its binary complex with an ATP analog has revealed an identical open kinase conformation. The secondary structure and side chain interactions stabilize the activation loop of Chk1 and enable kinase activity without phosphorylation of the catalytic domain. Molecular modeling of the interaction of a Cdc25C peptide with Chk1 has uncovered several conserved residues that are important for substrate selectivity. In addition, we found that the less conserved C-terminal region negatively impacts Chk1 kinase activity.

About this StructureAbout this Structure

1IA8 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

The 1.7 A crystal structure of human cell cycle checkpoint kinase Chk1: implications for Chk1 regulation., Chen P, Luo C, Deng Y, Ryan K, Register J, Margosiak S, Tempczyk-Russell A, Nguyen B, Myers P, Lundgren K, Kan CC, O'Connor PM, Cell. 2000 Mar 17;100(6):681-92. PMID:10761933

Page seeded by OCA on Thu Mar 20 11:47:37 2008

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OCA

@@ Line 1: / Line 1: @@
-[[Image:1ia8.gif|left|200px]]<br /><applet load="1ia8" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1ia8.gif|left|200px]]
-caption="1ia8, resolution 1.7&Aring;" />
-'''THE 1.7 A CRYSTAL STRUCTURE OF HUMAN CELL CYCLE CHECKPOINT KINASE CHK1'''<br />
+ {{Structure
+|PDB= 1ia8 |SIZE=350|CAPTION= <scene name='initialview01'>1ia8</scene>, resolution 1.7&Aring;
+|SITE=
+|LIGAND= <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene>
+|ACTIVITY=
+|GENE=
+}}
+'''THE 1.7 A CRYSTAL STRUCTURE OF HUMAN CELL CYCLE CHECKPOINT KINASE CHK1'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-IA8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IA8 OCA].
+IA8 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IA8 OCA].
 ==Reference==
-The 1.7 A crystal structure of human cell cycle checkpoint kinase Chk1: implications for Chk1 regulation., Chen P, Luo C, Deng Y, Ryan K, Register J, Margosiak S, Tempczyk-Russell A, Nguyen B, Myers P, Lundgren K, Kan CC, O'Connor PM, Cell. 2000 Mar 17;100(6):681-92. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10761933 10761933]
+The 1.7 A crystal structure of human cell cycle checkpoint kinase Chk1: implications for Chk1 regulation., Chen P, Luo C, Deng Y, Ryan K, Register J, Margosiak S, Tempczyk-Russell A, Nguyen B, Myers P, Lundgren K, Kan CC, O'Connor PM, Cell. 2000 Mar 17;100(6):681-92. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10761933 10761933]
 [[Category: Homo sapiens]]
 [[Category: Single protein]]
@@ Line 28: / Line 37: @@
 [[Category: protein kinase]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:09:41 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:47:37 2008''