1i9t: Difference between revisions
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[[Image:1i9t.gif|left|200px]] | [[Image:1i9t.gif|left|200px]] | ||
'''CRYSTAL STRUCTURE OF THE OXIDIZED RNA TRIPHOSPHATASE DOMAIN OF MOUSE MRNA CAPPING ENZYME''' | {{Structure | ||
|PDB= 1i9t |SIZE=350|CAPTION= <scene name='initialview01'>1i9t</scene>, resolution 1.7Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=CAC:CACODYLATE+ION'>CAC</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> and <scene name='pdbligand=IPA:ISOPROPYL ALCOHOL'>IPA</scene> | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/Polynucleotide_5'-phosphatase Polynucleotide 5'-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.33 3.1.3.33] | |||
|GENE= | |||
}} | |||
'''CRYSTAL STRUCTURE OF THE OXIDIZED RNA TRIPHOSPHATASE DOMAIN OF MOUSE MRNA CAPPING ENZYME''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1I9T is a [ | 1I9T is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I9T OCA]. | ||
==Reference== | ==Reference== | ||
Structure and mechanism of the RNA triphosphatase component of mammalian mRNA capping enzyme., Changela A, Ho CK, Martins A, Shuman S, Mondragon A, EMBO J. 2001 May 15;20(10):2575-86. PMID:[http:// | Structure and mechanism of the RNA triphosphatase component of mammalian mRNA capping enzyme., Changela A, Ho CK, Martins A, Shuman S, Mondragon A, EMBO J. 2001 May 15;20(10):2575-86. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11350947 11350947] | ||
[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
[[Category: Polynucleotide 5'-phosphatase]] | [[Category: Polynucleotide 5'-phosphatase]] | ||
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[[Category: rna triphosphatase domain]] | [[Category: rna triphosphatase domain]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:47:28 2008'' | ||
Revision as of 12:47, 20 March 2008
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| , resolution 1.7Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , and | ||||||
| Activity: | Polynucleotide 5'-phosphatase, with EC number 3.1.3.33 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF THE OXIDIZED RNA TRIPHOSPHATASE DOMAIN OF MOUSE MRNA CAPPING ENZYME
OverviewOverview
The 5' capping of mammalian pre-mRNAs is initiated by RNA triphosphatase, a member of the cysteine phosphatase superfamily. Here we report the 1.65 A crystal structure of mouse RNA triphosphatase, which reveals a deep, positively charged active site pocket that can fit a 5' triphosphate end. Structural, biochemical and mutational results show that despite sharing an HCxxxxxR(S/T) motif, a phosphoenzyme intermediate and a core alpha/beta-fold with other cysteine phosphatases, the mechanism of phosphoanhydride cleavage by mammalian capping enzyme differs from that used by protein phosphatases to hydrolyze phosphomonoesters. The most significant difference is the absence of a carboxylate general acid catalyst in RNA triphosphatase. Residues conserved uniquely among the RNA phosphatase subfamily are important for function in cap formation and are likely to play a role in substrate recognition.
About this StructureAbout this Structure
1I9T is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.
ReferenceReference
Structure and mechanism of the RNA triphosphatase component of mammalian mRNA capping enzyme., Changela A, Ho CK, Martins A, Shuman S, Mondragon A, EMBO J. 2001 May 15;20(10):2575-86. PMID:11350947
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