1i4w: Difference between revisions
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[[Image:1i4w.gif|left|200px]] | [[Image:1i4w.gif|left|200px]] | ||
'''THE CRYSTAL STRUCTURE OF THE TRANSCRIPTION FACTOR SC-MTTFB OFFERS INTRIGUING INSIGHTS INTO MITOCHONDRIAL TRANSCRIPTION''' | {{Structure | ||
|PDB= 1i4w |SIZE=350|CAPTION= <scene name='initialview01'>1i4w</scene>, resolution 2.60Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=XE:XENON'>XE</scene> | |||
|ACTIVITY= | |||
|GENE= MTF1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]) | |||
}} | |||
'''THE CRYSTAL STRUCTURE OF THE TRANSCRIPTION FACTOR SC-MTTFB OFFERS INTRIGUING INSIGHTS INTO MITOCHONDRIAL TRANSCRIPTION''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1I4W is a [ | 1I4W is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I4W OCA]. | ||
==Reference== | ==Reference== | ||
Crystal structure of the transcription factor sc-mtTFB offers insights into mitochondrial transcription., Schubot FD, Chen CJ, Rose JP, Dailey TA, Dailey HA, Wang BC, Protein Sci. 2001 Oct;10(10):1980-8. PMID:[http:// | Crystal structure of the transcription factor sc-mtTFB offers insights into mitochondrial transcription., Schubot FD, Chen CJ, Rose JP, Dailey TA, Dailey HA, Wang BC, Protein Sci. 2001 Oct;10(10):1980-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11567089 11567089] | ||
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: transcription initiation]] | [[Category: transcription initiation]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:45:35 2008'' | ||
Revision as of 12:45, 20 March 2008
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| , resolution 2.60Å | |||||||
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| Ligands: | |||||||
| Gene: | MTF1 (Saccharomyces cerevisiae) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
THE CRYSTAL STRUCTURE OF THE TRANSCRIPTION FACTOR SC-MTTFB OFFERS INTRIGUING INSIGHTS INTO MITOCHONDRIAL TRANSCRIPTION
OverviewOverview
Although it is commonly accepted that binding of mitochondrial transcription factor sc-mtTFB to the mitochondrial RNA polymerase is required for specific transcription initiation in Saccharomyces cerevisiae, its precise role has remained undefined. In the present work, the crystal structure of sc-mtTFB has been determined to 2.6 A resolution. The protein consists of two domains, an N-terminal alpha/beta-domain and a smaller domain made up of four alpha-helices. Contrary to previous predictions, sc-mtTFB does not resemble Escherichia coli sigma-factors but rather is structurally homologous to rRNA methyltransferase ErmC'. This suggests that sc-mtTFB functions as an RNA-binding protein, an observation standing in contradiction to the existing model, which proposed a direct interaction of sc-mtTFB with the mitochondrial DNA promoter. Based on the structure, we propose that the promoter specificity region is located on the mitochondrial RNA polymerase and that binding of sc-mtTFB indirectly mediates interaction of the core enzyme with the promoter site.
About this StructureAbout this Structure
1I4W is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of the transcription factor sc-mtTFB offers insights into mitochondrial transcription., Schubot FD, Chen CJ, Rose JP, Dailey TA, Dailey HA, Wang BC, Protein Sci. 2001 Oct;10(10):1980-8. PMID:11567089
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