2glh: Difference between revisions

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-[[Image:2glh.png|left|200px]]
+==Solution Conformation of Salmon Calcitonin in Sodium Dodecyl Sulfate Micelles==
+<StructureSection load='2glh' size='340' side='right' caption='[[2glh]], [[NMR_Ensembles_of_Models | 100 NMR models]]' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[2glh]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GLH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2GLH FirstGlance]. <br>
+</td></tr><tr><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
+<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2glg|2glg]]</td></tr>
+<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2glh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2glh OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2glh RCSB], [http://www.ebi.ac.uk/pdbsum/2glh PDBsum]</span></td></tr>
+<table>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gl/2glh_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Salmon calcitonin (sCT) forms an amphipathic helix in the region 9-19, with the C-terminal decapeptide interacting with the helix (Amodeo, P., Motta, A., Strazzullo, G., Castiglione Morelli, M. A. (1999) J. Biomol. NMR 13, 161-174). To uncover the structural requirements for the hormone bioactivity, we investigated several sCT analogs. They were designed so as to alter the length of the central helix by removal and/or replacement of flanking residues and by selectively mutating or deleting residues inside the helix. The helix content was assessed by circular dichroism and NMR spectroscopies; the receptor binding affinity in human breast cancer cell line T 47D and the in vivo hypocalcemic activity were also evaluated. In particular, by NMR spectroscopy and molecular dynamics calculations we studied Leu(23),Ala(24)-sCT in which Pro(23) and Arg(24) were replaced by helix inducing residues. Compared with sCT, it assumes a longer amphipathic alpha-helix, with decreased binding affinity and one-fifth of the hypocalcemic activity, therefore supporting the idea of a relationship between a definite helix length and bioactivity. From the analysis of other sCT mutants, we inferred that the correct helix length is located in the 9-19 region and requires long range interactions and the presence of specific regions of residues within the sequence for high binding affinity and hypocalcemic activity. Taken together, the structural and biological data identify well defined structural parameters of the helix for sCT bioactivity.
-{{STRUCTURE_2glh|  PDB=2glh  |  SCENE=  }}
+Structural determinants of salmon calcitonin bioactivity: the role of the Leu-based amphipathic alpha-helix.,Andreotti G, Mendez BL, Amodeo P, Morelli MA, Nakamuta H, Motta A J Biol Chem. 2006 Aug 25;281(34):24193-203. Epub 2006 Jun 9. PMID:16766525<ref>PMID:16766525</ref>
-===Solution Conformation of Salmon Calcitonin in Sodium Dodecyl Sulfate Micelles===
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-{{ABSTRACT_PUBMED_16766525}}
+== References ==
+<references/>
-==About this Structure==
+__TOC__
-[[2glh]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GLH OCA].
+</StructureSection>
-==Reference==
-<ref group="xtra">PMID:016766525</ref><references group="xtra"/>
 [[Category: Amodeo, P.]]
 [[Category: Andreotti, G.]]