1huy: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:1huy.jpg|left|200px]] | [[Image:1huy.jpg|left|200px]] | ||
'''CRYSTAL STRUCTURE OF CITRINE, AN IMPROVED YELLOW VARIANT OF GREEN FLUORESCENT PROTEIN''' | {{Structure | ||
|PDB= 1huy |SIZE=350|CAPTION= <scene name='initialview01'>1huy</scene>, resolution 2.20Å | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= | |||
|GENE= GFP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6100 Aequorea victoria]) | |||
}} | |||
'''CRYSTAL STRUCTURE OF CITRINE, AN IMPROVED YELLOW VARIANT OF GREEN FLUORESCENT PROTEIN''' | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
1HUY is a [ | 1HUY is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HUY OCA]. | ||
==Reference== | ==Reference== | ||
Reducing the environmental sensitivity of yellow fluorescent protein. Mechanism and applications., Griesbeck O, Baird GS, Campbell RE, Zacharias DA, Tsien RY, J Biol Chem. 2001 Aug 3;276(31):29188-94. Epub 2001 May 31. PMID:[http:// | Reducing the environmental sensitivity of yellow fluorescent protein. Mechanism and applications., Griesbeck O, Baird GS, Campbell RE, Zacharias DA, Tsien RY, J Biol Chem. 2001 Aug 3;276(31):29188-94. Epub 2001 May 31. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11387331 11387331] | ||
[[Category: Aequorea victoria]] | [[Category: Aequorea victoria]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 21: | Line 30: | ||
[[Category: chromophore]] | [[Category: chromophore]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:41:54 2008'' | ||
Revision as of 12:41, 20 March 2008
| |||||||
| , resolution 2.20Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | GFP (Aequorea victoria) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF CITRINE, AN IMPROVED YELLOW VARIANT OF GREEN FLUORESCENT PROTEIN
OverviewOverview
Yellow mutants of the green fluorescent protein (YFP) are crucial constituents of genetically encoded indicators of signal transduction and fusions to monitor protein-protein interactions. However, previous YFPs show excessive pH sensitivity, chloride interference, poor photostability, or poor expression at 37 degrees C. Protein evolution in Escherichia coli has produced a new YFP named Citrine, in which the mutation Q69M confers a much lower pK(a) (5.7) than for previous YFPs, indifference to chloride, twice the photostability of previous YFPs, and much better expression at 37 degrees C and in organelles. The halide resistance is explained by a 2.2-A x-ray crystal structure of Citrine, showing that the methionine side chain fills what was once a large halide-binding cavity adjacent to the chromophore. Insertion of calmodulin within Citrine or fusion of cyan fluorescent protein, calmodulin, a calmodulin-binding peptide and Citrine has generated improved calcium indicators. These chimeras can be targeted to multiple cellular locations and have permitted the first single-cell imaging of free [Ca(2+)] in the Golgi. Citrine is superior to all previous YFPs except when pH or halide sensitivity is desired and is particularly advantageous within genetically encoded fluorescent indicators of physiological signals.
About this StructureAbout this Structure
1HUY is a Single protein structure of sequence from Aequorea victoria. Full crystallographic information is available from OCA.
ReferenceReference
Reducing the environmental sensitivity of yellow fluorescent protein. Mechanism and applications., Griesbeck O, Baird GS, Campbell RE, Zacharias DA, Tsien RY, J Biol Chem. 2001 Aug 3;276(31):29188-94. Epub 2001 May 31. PMID:11387331
Page seeded by OCA on Thu Mar 20 11:41:54 2008