1hmh: Difference between revisions
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'''THREE-DIMENSIONAL STRUCTURE OF A HAMMERHEAD RIBOZYME''' | {{Structure | ||
|PDB= 1hmh |SIZE=350|CAPTION= <scene name='initialview01'>1hmh</scene>, resolution 2.600Å | |||
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'''THREE-DIMENSIONAL STRUCTURE OF A HAMMERHEAD RIBOZYME''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1HMH is a [ | 1HMH is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HMH OCA]. | ||
==Reference== | ==Reference== | ||
Three-dimensional structure of a hammerhead ribozyme., Pley HW, Flaherty KM, McKay DB, Nature. 1994 Nov 3;372(6501):68-74. PMID:[http:// | Three-dimensional structure of a hammerhead ribozyme., Pley HW, Flaherty KM, McKay DB, Nature. 1994 Nov 3;372(6501):68-74. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7969422 7969422] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Flaherty, K M.]] | [[Category: Flaherty, K M.]] | ||
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[[Category: loop]] | [[Category: loop]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:39:03 2008'' | ||
Revision as of 12:39, 20 March 2008
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THREE-DIMENSIONAL STRUCTURE OF A HAMMERHEAD RIBOZYME
OverviewOverview
The hammerhead ribozyme is a small catalytic RNA motif made up of three base-paired stems and a core of highly conserved, non-complementary nucleotides essential for catalysis. The X-ray crystallographic structure of a hammerhead RNA-DNA ribozyme-inhibitor complex at 2.6 A resolution reveals that the base-paired stems are A-form helices and that the core has two structural domains. The first domain is formed by the sequence 5'-CUGA following stem I and is a sharp turn identical to the uridine turn of transfer RNA, whereas the second is a non-Watson-Crick three-base-pair duplex with a divalent-ion binding site. The phosphodiester backbone of the DNA inhibitor strand is splayed out at the phosphate 5' to the cleavage site. The structure indicates that the ribozyme may destabilize a substrate strand in order to facilitate twisting of the substrate to allow cleavage of the scissile bond.
About this StructureAbout this Structure
1HMH is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
ReferenceReference
Three-dimensional structure of a hammerhead ribozyme., Pley HW, Flaherty KM, McKay DB, Nature. 1994 Nov 3;372(6501):68-74. PMID:7969422
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