1hl6: Difference between revisions

Revision as of 12:38, 20 March 2008

, resolution 2.5Å

Coordinates:

A NOVEL MODE OF RBD-PROTEIN RECOGNITION IN THE Y14-MAGO COMPLEX

OverviewOverview

Y14 and Mago are conserved eukaryotic proteins that associate with spliced mRNAs in the nucleus and remain associated at exon junctions during and after nuclear export. In the cytoplasm, Y14 is involved in mRNA quality control via the nonsense-mediated mRNA decay (NMD) pathway and, together with Mago, is involved in localization of osk (oskar) mRNA. We have determined the crystal structure of the complex between Drosophila melanogaster Y14 and Mago at a resolution of 2.5 A. The structure reveals an atypical mode of protein-protein recognition mediated by an RNA-binding domain (RBD). Instead of binding RNA, the RBD of Y14 engages its RNP1 and RNP2 motifs to bind Mago. Using structure-guided mutagenesis, we show that Mago is also a component of the NMD pathway, and that its association with Y14 is essential for function. Heterodimerization creates a single structural platform that interacts with the NMD machinery via phylogenetically conserved residues.

About this StructureAbout this Structure

1HL6 is a Protein complex structure of sequences from Drosophila melanogaster. Full crystallographic information is available from OCA.

ReferenceReference

A novel mode of RBD-protein recognition in the Y14-Mago complex., Fribourg S, Gatfield D, Izaurralde E, Conti E, Nat Struct Biol. 2003 Jun;10(6):433-9. PMID:12730685

Page seeded by OCA on Thu Mar 20 11:38:37 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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-[[Image:1hl6.jpg|left|200px]]<br /><applet load="1hl6" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1hl6.jpg|left|200px]]
-caption="1hl6, resolution 2.5&Aring;" />
-'''A NOVEL MODE OF RBD-PROTEIN RECOGNITION IN THE Y14-MAGO COMPLEX'''<br />
+ {{Structure
+|PDB= 1hl6 |SIZE=350|CAPTION= <scene name='initialview01'>1hl6</scene>, resolution 2.5&Aring;
+|SITE=
+|LIGAND=
+|ACTIVITY=
+|GENE=
+}}
+'''A NOVEL MODE OF RBD-PROTEIN RECOGNITION IN THE Y14-MAGO COMPLEX'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-HL6 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HL6 OCA].
+HL6 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HL6 OCA].
 ==Reference==
-A novel mode of RBD-protein recognition in the Y14-Mago complex., Fribourg S, Gatfield D, Izaurralde E, Conti E, Nat Struct Biol. 2003 Jun;10(6):433-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12730685 12730685]
+A novel mode of RBD-protein recognition in the Y14-Mago complex., Fribourg S, Gatfield D, Izaurralde E, Conti E, Nat Struct Biol. 2003 Jun;10(6):433-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12730685 12730685]
 [[Category: Drosophila melanogaster]]
 [[Category: Protein complex]]
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 [[Category: rnp]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:02:24 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:38:37 2008''