1him: Difference between revisions

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File:1him.gif

, resolution 2.9Å

Ligands:

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STRUCTURAL EVIDENCE FOR INDUCED FIT AS A MECHANISM FOR ANTIBODY-ANTIGEN RECOGNITION

OverviewOverview

The three-dimensional structure of a specific antibody (Fab 17/9) to a peptide immunogen from influenza virus hemagglutinin [HA1(75-110)] and two independent crystal complexes of this antibody with bound peptide (TyrP100-LeuP108) have been determined by x-ray crystallographic techniques at 2.0 A, 2.9 A, and 3.1 A resolution, respectively. The nonapeptide antigen assumes a type I beta turn in the antibody combining site and interacts primarily with the Fab hypervariable loops L3, H2, and H3. Comparison of the bound and unbound Fab structures shows that a major rearrangement in the H3 loop accompanies antigen binding. This conformational change results in the creation of a binding pocket for the beta turn of the peptide, allowing TyrP105 to be accommodated. The conformation of the peptide bound to the antibody shows similarity to its cognate sequence in the HA1, suggesting a possible mechanism for the cross-reactivity of this Fab with monomeric hemagglutinin. The structures of the free and antigen bound antibodies demonstrate the flexibility of the antibody combining site and provide an example of induced fit as a mechanism for antibody-antigen recognition.

About this StructureAbout this Structure

1HIM is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.

ReferenceReference

Structural evidence for induced fit as a mechanism for antibody-antigen recognition., Rini JM, Schulze-Gahmen U, Wilson IA, Science. 1992 Feb 21;255(5047):959-65. PMID:1546293

Page seeded by OCA on Thu Mar 20 11:37:33 2008

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OCA

@@ Line 1: / Line 1: @@
-[[Image:1him.gif|left|200px]]<br /><applet load="1him" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1him.gif|left|200px]]
-caption="1him, resolution 2.9&Aring;" />
-'''STRUCTURAL EVIDENCE FOR INDUCED FIT AS A MECHANISM FOR ANTIBODY-ANTIGEN RECOGNITION'''<br />
+ {{Structure
+|PDB= 1him |SIZE=350|CAPTION= <scene name='initialview01'>1him</scene>, resolution 2.9&Aring;
+|SITE=
+|LIGAND= <scene name='pdbligand=NH2:AMINO GROUP'>NH2</scene>
+|ACTIVITY=
+|GENE=
+}}
+'''STRUCTURAL EVIDENCE FOR INDUCED FIT AS A MECHANISM FOR ANTIBODY-ANTIGEN RECOGNITION'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-HIM is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=NH2:'>NH2</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HIM OCA].
+HIM is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HIM OCA].
 ==Reference==
-Structural evidence for induced fit as a mechanism for antibody-antigen recognition., Rini JM, Schulze-Gahmen U, Wilson IA, Science. 1992 Feb 21;255(5047):959-65. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=1546293 1546293]
+Structural evidence for induced fit as a mechanism for antibody-antigen recognition., Rini JM, Schulze-Gahmen U, Wilson IA, Science. 1992 Feb 21;255(5047):959-65. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/1546293 1546293]
 [[Category: Protein complex]]
 [[Category: Schulze-Gahmen, U.]]
@@ Line 17: / Line 26: @@
 [[Category: immunoglobulin]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:01:35 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:37:33 2008''