1hcm: Difference between revisions
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[[Image:1hcm.gif|left|200px]] | [[Image:1hcm.gif|left|200px]] | ||
'''CYTOCHROME CD1 NITRITE REDUCTASE, OXIDISED FROM FROM TETRAGONAL CRYSTALS''' | {{Structure | ||
|PDB= 1hcm |SIZE=350|CAPTION= <scene name='initialview01'>1hcm</scene>, resolution 2.5Å | |||
|SITE= <scene name='pdbsite=C1A:The+C+Hem+Is+The+Point+Of+Electron+Entry'>C1A</scene>, <scene name='pdbsite=C1B:The+C+Hem+Is+The+Point+Of+Electron+Entry'>C1B</scene>, <scene name='pdbsite=D1A:The+D+Hem+Is+The+Site+Of+Catalytic+Activity'>D1A</scene> and <scene name='pdbsite=D1B:The+D+Hem+Is+The+Site+Of+Catalytic+Activity'>D1B</scene> | |||
|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=HEC:HEME+C'>HEC</scene> and <scene name='pdbligand=DHE:HEME D'>DHE</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''CYTOCHROME CD1 NITRITE REDUCTASE, OXIDISED FROM FROM TETRAGONAL CRYSTALS''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1HCM is a [ | 1HCM is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Paracoccus_pantotrophus Paracoccus pantotrophus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HCM OCA]. | ||
==Reference== | ==Reference== | ||
The Structure of an alternative form of Paracoccus pantotrophus cytochrome cd(1) nitrite reductase., Sjogren T, Hajdu J, J Biol Chem. 2001 Aug 3;276(31):29450-5. Epub 2001 May 23. PMID:[http:// | The Structure of an alternative form of Paracoccus pantotrophus cytochrome cd(1) nitrite reductase., Sjogren T, Hajdu J, J Biol Chem. 2001 Aug 3;276(31):29450-5. Epub 2001 May 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11373294 11373294] | ||
[[Category: Paracoccus pantotrophus]] | [[Category: Paracoccus pantotrophus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: oxidoreductase]] | [[Category: oxidoreductase]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:35:19 2008'' | ||
Revision as of 12:35, 20 March 2008
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| , resolution 2.5Å | |||||||
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| Sites: | , , and | ||||||
| Ligands: | , and | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CYTOCHROME CD1 NITRITE REDUCTASE, OXIDISED FROM FROM TETRAGONAL CRYSTALS
OverviewOverview
Cytochrome cd(1) nitrite reductase is a bifunctional enzyme, which can catalyze the 1-electron reduction of nitrite to nitric oxide and the 4-electron reduction of dioxygen to water. Here we describe the structure of reduced nitrite reductase, crystallized under anaerobic conditions. The structure reveals substantial domain rearrangements with the c domain rotated by 60 degrees and shifted by approximately 20 A compared with previously known structures from crystals grown under oxidizing conditions. This alternative conformation gives rise to different electron transfer routes between the c and d(1) domains and points to the involvement of elements of very large structural changes in the function in this enzyme. In the present structure, the c heme has a His-69/Met-106 ligation, and this ligation does not change upon oxidation in the crystal. The d(1) heme is penta-coordinated, and the d(1) iron is displaced from the heme plane by 0.5 A toward the proximal ligand, His-200. After oxidation, the iron moves into the d(1) heme plane. A surprising finding is that although reduced nitrite reductase can be readily oxidized by dioxygen in the new crystal, it cannot turnover with its other substrate, nitrite. The results suggest that the rearrangement of the domains affects catalysis and substrate selectivity.
About this StructureAbout this Structure
1HCM is a Single protein structure of sequence from Paracoccus pantotrophus. Full crystallographic information is available from OCA.
ReferenceReference
The Structure of an alternative form of Paracoccus pantotrophus cytochrome cd(1) nitrite reductase., Sjogren T, Hajdu J, J Biol Chem. 2001 Aug 3;276(31):29450-5. Epub 2001 May 23. PMID:11373294
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