1gx4: Difference between revisions

← Older edit Newer edit →

Revision as of 12:29, 20 March 2008

File:1gx4.gif

, resolution 1.46Å

Sites:

Ligands:

, and

Activity:

Transferred entry: 2.4.1.87, with EC number 2.4.1.151

Coordinates:

save as pdb, mmCIF, xml

ALPHA-,1,3 GALACTOSYLTRANSFERASE- N-ACETYL LACTOSAMINE COMPLEX

OverviewOverview

Bovine alpha-1,3-galactosyltransferase (alpha3GT) catalyzes the synthesis of the alpha-galactose (alpha-Gal) epitope, the target of natural human antibodies. It represents a family of enzymes, including the histo blood group A and B transferases, that catalyze retaining glycosyltransfer reactions of unknown mechanism. An initial study of alpha3GT in a crystal form with limited resolution and considerable disorder suggested the possible formation of a beta-galactosyl-enzyme covalent intermediate (Gastinel, L. N., Bignon, C., Misra, A. K., Hindsgaul, O., Shaper, J. H., and Joziasse, D. H. (2001) EMBO J. 20, 638-649). Highly ordered structures are described for complexes of alpha3GT with donor substrate, UDP-galactose, UDP- glucose, and two acceptor substrates, lactose and N-acetyllactosamine, at resolutions up to 1.46 A. Structural and calorimetric binding studies suggest an obligatory ordered binding of donor and acceptor substrates, linked to a donor substrate-induced conformational change, and the direct participation of UDP in acceptor binding. The monosaccharide-UDP bond is cleaved in the structures containing UDP-galactose and UDP-glucose, producing non-covalent complexes containing buried beta-galactose and alpha-glucose. The location of these monosaccharides and molecular modeling suggest that binding of a distorted conformation of UDP-galactose may be important in the catalytic mechanism of alpha3GT.

About this StructureAbout this Structure

1GX4 is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.

ReferenceReference

Structural basis of ordered binding of donor and acceptor substrates to the retaining glycosyltransferase, alpha-1,3-galactosyltransferase., Boix E, Zhang Y, Swaminathan GJ, Brew K, Acharya KR, J Biol Chem. 2002 Aug 2;277(31):28310-8. Epub 2002 May 14. PMID:12011052

Page seeded by OCA on Thu Mar 20 11:29:12 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1gx4.gif|left|200px]]<br /><applet load="1gx4" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1gx4.gif|left|200px]]
-caption="1gx4, resolution 1.46&Aring;" />
-'''ALPHA-,1,3 GALACTOSYLTRANSFERASE- N-ACETYL LACTOSAMINE COMPLEX'''<br />
+ {{Structure
+|PDB= 1gx4 |SIZE=350|CAPTION= <scene name='initialview01'>1gx4</scene>, resolution 1.46&Aring;
+|SITE= <scene name='pdbsite=AC1:Nag+Binding+Site+For+Chain+B'>AC1</scene>
+|LIGAND= <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=UDP:URIDINE-5'-DIPHOSPHATE'>UDP</scene> and <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>
+|ACTIVITY= [http://en.wikipedia.org/wiki/Transferred_entry:_2.4.1.87 Transferred entry: 2.4.1.87], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.151 2.4.1.151]
+|GENE=
+}}
+'''ALPHA-,1,3 GALACTOSYLTRANSFERASE- N-ACETYL LACTOSAMINE COMPLEX'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-GX4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with <scene name='pdbligand=MN:'>MN</scene>, <scene name='pdbligand=UDP:'>UDP</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Transferred_entry:_2.4.1.87 Transferred entry: 2.4.1.87], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.151 2.4.1.151] Known structural/functional Site: <scene name='pdbsite=AC1:Nag+Binding+Site+For+Chain+B'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GX4 OCA].
+GX4 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GX4 OCA].
 ==Reference==
-Structural basis of ordered binding of donor and acceptor substrates to the retaining glycosyltransferase, alpha-1,3-galactosyltransferase., Boix E, Zhang Y, Swaminathan GJ, Brew K, Acharya KR, J Biol Chem. 2002 Aug 2;277(31):28310-8. Epub 2002 May 14. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12011052 12011052]
+Structural basis of ordered binding of donor and acceptor substrates to the retaining glycosyltransferase, alpha-1,3-galactosyltransferase., Boix E, Zhang Y, Swaminathan GJ, Brew K, Acharya KR, J Biol Chem. 2002 Aug 2;277(31):28310-8. Epub 2002 May 14. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12011052 12011052]
 [[Category: Bos taurus]]
 [[Category: Single protein]]
@@ Line 22: / Line 31: @@
 [[Category: MN]]
 [[Category: UDP]]
-[[Category: blood group sugars]]
+[[Category: blood group sugar]]
 [[Category: galactosyltransferase]]
 [[Category: glycosyltransferase]]
@@ Line 29: / Line 38: @@
 [[Category: transferase]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:54:56 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:29:12 2008''