1gv3: Difference between revisions

Revision as of 12:28, 20 March 2008

, resolution 2.00Å

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Ligands:

Coordinates:

THE 2.0 ANGSTROM RESOLUTION STRUCTURE OF THE CATALYTIC PORTION OF A CYANOBACTERIAL MEMBRANE-BOUND MANGANESE SUPEROXIDE DISMUTASE

OverviewOverview

Cyanobacteria are shown to be unique in containing membrane-bound manganese superoxide dismutases (MnSOD). They are homodimeric type 2 membrane proteins that protect this phototrophic organism against oxidative stress. We have determined, for the first time, the 2.0A resolution structure of the catalytic portion of the MnSOD from the filamentous cyanobacterium Anabaena PCC 7120. Within each subunit, both the N-terminal helical hairpin (His94 and His145) and the C-terminal alpha/beta domain (His232 and Asp228) contribute ligands to the catalytic manganese site. Together with a water or hydroxide ion (OH(x)) a five-coordinated trigonal bipyramidal geometry is formed, with OH(x) and His90 forming the axial ligands and manganese shifted out of the equatorial plane in the direction of OH(x). The ligands including OH(x) are tightly constrained by hydrogen bonding with surrounding residues either from the same monomer (Tyr98, Asn144, Trp194, Gln213, Val229, Trp230) or from the neighbouring subunit (Glu231, Tyr235). This underlines the important role of the symmetric dimeric structure of MnSODs in contributing elements to both the active site and the substrate funnel. The Mn cdots, three dots, centered Mn distance (18.4A) is bridged by the hydrogen-bonded His232 of one monomer with Glu231 of the other monomer. A detailed discussion of the structure, a comparison with known structures of soluble MnSODs as well as a model of the cyanobacterial membrane-bound MnSOD is presented.

About this StructureAbout this Structure

1GV3 is a Single protein structure of sequence from Anabaena sp.. Full crystallographic information is available from OCA.

ReferenceReference

The 2.0A resolution structure of the catalytic portion of a cyanobacterial membrane-bound manganese superoxide dismutase., Atzenhofer W, Regelsberger G, Jacob U, Peschek G, Furtmuller P, Huber R, Obinger C, J Mol Biol. 2002 Aug 16;321(3):479-89. PMID:12162960

Page seeded by OCA on Thu Mar 20 11:28:28 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1gv3.jpg|left|200px]]<br /><applet load="1gv3" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1gv3.jpg|left|200px]]
-caption="1gv3, resolution 2.00&Aring;" />
-'''THE 2.0 ANGSTROM RESOLUTION STRUCTURE OF THE CATALYTIC PORTION OF A CYANOBACTERIAL MEMBRANE-BOUND MANGANESE SUPEROXIDE DISMUTASE'''<br />
+ {{Structure
+|PDB= 1gv3 |SIZE=350|CAPTION= <scene name='initialview01'>1gv3</scene>, resolution 2.00&Aring;
+|SITE= <scene name='pdbsite=AC1:Mn+Binding+Site+For+Chain+B'>AC1</scene>
+|LIGAND= <scene name='pdbligand=MN:MANGANESE (II) ION'>MN</scene>
+|ACTIVITY=
+|GENE=
+}}
+'''THE 2.0 ANGSTROM RESOLUTION STRUCTURE OF THE CATALYTIC PORTION OF A CYANOBACTERIAL MEMBRANE-BOUND MANGANESE SUPEROXIDE DISMUTASE'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-GV3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Anabaena_sp. Anabaena sp.] with <scene name='pdbligand=MN:'>MN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=AC1:Mn+Binding+Site+For+Chain+B'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GV3 OCA].
+GV3 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Anabaena_sp. Anabaena sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GV3 OCA].
 ==Reference==
-The 2.0A resolution structure of the catalytic portion of a cyanobacterial membrane-bound manganese superoxide dismutase., Atzenhofer W, Regelsberger G, Jacob U, Peschek G, Furtmuller P, Huber R, Obinger C, J Mol Biol. 2002 Aug 16;321(3):479-89. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12162960 12162960]
+The 2.0A resolution structure of the catalytic portion of a cyanobacterial membrane-bound manganese superoxide dismutase., Atzenhofer W, Regelsberger G, Jacob U, Peschek G, Furtmuller P, Huber R, Obinger C, J Mol Biol. 2002 Aug 16;321(3):479-89. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12162960 12162960]
 [[Category: Anabaena sp.]]
 [[Category: Single protein]]
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 [[Category: manganese superoxide dismutase]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:54:20 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:28:28 2008''