1gtt: Difference between revisions

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CRYSTAL STRUCTURE OF HPCE

OverviewOverview

The structure of the bifunctional enzyme HpcE (OPET decarboxylase/HHDD isomerase) from Escherichia coli shows that the protein consists of highly similar N and C terminal halves. Sequence matches suggest that this fold is widespread among different species, including man. Many of these homologues are uncharacterized but apparently connected with the metabolism of aromatic compounds. The domain shows similar topology to the C terminal domain of fumarylacetoacetate hydrolase (FAH), a functionally related enzyme, despite lacking significant overall sequence similarity. HpcE is known to catalyze two rather different reactions, and comparisons with FAH allow some tentative conclusions to be drawn about the active sites. Key mutations within the active site apparently allow enzymes with this fold to carry out a variety chemical processes.

About this StructureAbout this Structure

1GTT is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

ReferenceReference

The crystal structure of HpcE, a bifunctional decarboxylase/isomerase with a multifunctional fold., Tame JR, Namba K, Dodson EJ, Roper DI, Biochemistry. 2002 Mar 5;41(9):2982-9. PMID:11863436

Page seeded by OCA on Thu Mar 20 11:27:47 2008

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OCA

@@ Line 1: / Line 1: @@
-[[Image:1gtt.gif|left|200px]]<br /><applet load="1gtt" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1gtt.gif|left|200px]]
-caption="1gtt, resolution 1.70&Aring;" />
-'''CRYSTAL STRUCTURE OF HPCE'''<br />
+ {{Structure
+|PDB= 1gtt |SIZE=350|CAPTION= <scene name='initialview01'>1gtt</scene>, resolution 1.70&Aring;
+|SITE= <scene name='pdbsite=CA1:Ca+Binding+Site+For+Chain+D'>CA1</scene>
+|LIGAND= <scene name='pdbligand=CA:CALCIUM ION'>CA</scene>
+|ACTIVITY=
+|GENE=
+}}
+'''CRYSTAL STRUCTURE OF HPCE'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-GTT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=CA1:Ca+Binding+Site+For+Chain+D'>CA1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GTT OCA].
+GTT is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GTT OCA].
 ==Reference==
-The crystal structure of HpcE, a bifunctional decarboxylase/isomerase with a multifunctional fold., Tame JR, Namba K, Dodson EJ, Roper DI, Biochemistry. 2002 Mar 5;41(9):2982-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11863436 11863436]
+The crystal structure of HpcE, a bifunctional decarboxylase/isomerase with a multifunctional fold., Tame JR, Namba K, Dodson EJ, Roper DI, Biochemistry. 2002 Mar 5;41(9):2982-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11863436 11863436]
 [[Category: Escherichia coli]]
 [[Category: Single protein]]
@@ Line 24: / Line 33: @@
 [[Category: multifunctional enzyme decarboxylase]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:53:59 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:27:47 2008''