1gtf: Difference between revisions
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[[Image:1gtf.jpg|left|200px]] | [[Image:1gtf.jpg|left|200px]] | ||
'''THE STRUCTURE OF THE TRP RNA-BINDING ATTENUATION PROTEIN (TRAP) BOUND TO A 53-NUCLEOTIDE RNA MOLECULE CONTAINING GAGUU REPEATS''' | {{Structure | ||
|PDB= 1gtf |SIZE=350|CAPTION= <scene name='initialview01'>1gtf</scene>, resolution 1.750Å | |||
|SITE= <scene name='pdbsite=AC1:TRP+Binding+Site+For+Chain+A'>AC1</scene>, <scene name='pdbsite=AC2:TRP+Binding+Site+For+Chain+B'>AC2</scene>, <scene name='pdbsite=AC3:TRP+Binding+Site+For+Chain+C'>AC3</scene>, <scene name='pdbsite=AC4:TRP+Binding+Site+For+Chain+D'>AC4</scene>, <scene name='pdbsite=AC5:TRP+Binding+Site+For+Chain+E'>AC5</scene>, <scene name='pdbsite=AC6:TRP+Binding+Site+For+Chain+F'>AC6</scene>, <scene name='pdbsite=AC7:TRP+Binding+Site+For+Chain+G'>AC7</scene>, <scene name='pdbsite=AC8:TRP+Binding+Site+For+Chain+H'>AC8</scene>, <scene name='pdbsite=AC9:TRP+Binding+Site+For+Chain+I'>AC9</scene>, <scene name='pdbsite=BC1:TRP+Binding+Site+For+Chain+J'>BC1</scene>, <scene name='pdbsite=BC2:TRP+Binding+Site+For+Chain+K'>BC2</scene>, <scene name='pdbsite=BC3:TRP+Binding+Site+For+Chain+L'>BC3</scene>, <scene name='pdbsite=BC4:TRP+Binding+Site+For+Chain+M'>BC4</scene>, <scene name='pdbsite=BC5:TRP+Binding+Site+For+Chain+N'>BC5</scene>, <scene name='pdbsite=BC6:TRP+Binding+Site+For+Chain+O'>BC6</scene>, <scene name='pdbsite=BC7:TRP+Binding+Site+For+Chain+P'>BC7</scene>, <scene name='pdbsite=BC8:TRP+Binding+Site+For+Chain+Q'>BC8</scene>, <scene name='pdbsite=BC9:TRP+Binding+Site+For+Chain+R'>BC9</scene>, <scene name='pdbsite=CC1:TRP+Binding+Site+For+Chain+S'>CC1</scene>, <scene name='pdbsite=CC2:TRP+Binding+Site+For+Chain+T'>CC2</scene>, <scene name='pdbsite=CC3:TRP+Binding+Site+For+Chain+U'>CC3</scene> and <scene name='pdbsite=CC4:TRP+Binding+Site+For+Chain+V'>CC4</scene> | |||
|LIGAND= <scene name='pdbligand=TRP:TRYPTOPHAN'>TRP</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''THE STRUCTURE OF THE TRP RNA-BINDING ATTENUATION PROTEIN (TRAP) BOUND TO A 53-NUCLEOTIDE RNA MOLECULE CONTAINING GAGUU REPEATS''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1GTF is a [ | 1GTF is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GTF OCA]. | ||
==Reference== | ==Reference== | ||
Specificity of TRAP-RNA interactions: crystal structures of two complexes with different RNA sequences., Hopcroft NH, Wendt AL, Gollnick P, Antson AA, Acta Crystallogr D Biol Crystallogr. 2002 Apr;58(Pt 4):615-21. Epub 2002, Mar 22. PMID:[http:// | Specificity of TRAP-RNA interactions: crystal structures of two complexes with different RNA sequences., Hopcroft NH, Wendt AL, Gollnick P, Antson AA, Acta Crystallogr D Biol Crystallogr. 2002 Apr;58(Pt 4):615-21. Epub 2002, Mar 22. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11914485 11914485] | ||
[[Category: Geobacillus stearothermophilus]] | [[Category: Geobacillus stearothermophilus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: trp rna]] | [[Category: trp rna]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:27:44 2008'' | ||
Revision as of 12:27, 20 March 2008
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| , resolution 1.750Å | |||||||
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| Sites: | , , , , , , , , , , , , , , , , , , , , and | ||||||
| Ligands: | |||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
THE STRUCTURE OF THE TRP RNA-BINDING ATTENUATION PROTEIN (TRAP) BOUND TO A 53-NUCLEOTIDE RNA MOLECULE CONTAINING GAGUU REPEATS
OverviewOverview
The trp RNA-binding attenuation protein (TRAP) regulates expression of the tryptophan biosynthetic genes in bacilli by binding to the leader region of the nascent trp operon mRNA. When activated by binding tryptophan, the 11-subunit circular TRAP molecule binds to a target sequence consisting of 11 (G/U)AG repeats, separated by two or three variable 'spacer' nucleotides. Reported here are two crystal structures of TRAP bound to RNAs containing 11 GAG repeats separated by UU and CC spacer nucleotides, determined at 1.75 and 2.50 A resolution, respectively. These show the spacer regions of the RNA molecules to be highly flexible, making no direct hydrogen-bonding contacts with the protein. Comparison of these structures with the previous structure of TRAP bound to (GAGAU)(10)GAG RNA, in which the spacer nucleotides stack with each other close to the protein surface, shows that the RNA can adopt different conformations depending on the sequence of the spacer regions. This gives insight into the structural basis of the specificity of TRAP and into the mechanism of binding.
About this StructureAbout this Structure
1GTF is a Single protein structure of sequence from Geobacillus stearothermophilus. Full crystallographic information is available from OCA.
ReferenceReference
Specificity of TRAP-RNA interactions: crystal structures of two complexes with different RNA sequences., Hopcroft NH, Wendt AL, Gollnick P, Antson AA, Acta Crystallogr D Biol Crystallogr. 2002 Apr;58(Pt 4):615-21. Epub 2002, Mar 22. PMID:11914485
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