2uxa: Difference between revisions

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-[[Image:2uxa.png|left|200px]]
+==CRYSTAL STRUCTURE OF THE GLUR2-FLIP LIGAND BINDING DOMAIN, R/G UNEDITED.==
+<StructureSection load='2uxa' size='340' side='right' caption='[[2uxa]], [[Resolution|resolution]] 2.38&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[2uxa]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2UXA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2UXA FirstGlance]. <br>
+</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GLU:GLUTAMIC+ACID'>GLU</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>
+<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1ftj|1ftj]], [[1ftk|1ftk]], [[1ftl|1ftl]], [[1ftm|1ftm]], [[1fto|1fto]], [[1fw0|1fw0]], [[1gr2|1gr2]], [[1lb8|1lb8]], [[1lb9|1lb9]], [[1lbb|1lbb]], [[1lbc|1lbc]], [[1m5b|1m5b]], [[1m5c|1m5c]], [[1m5d|1m5d]], [[1m5e|1m5e]], [[1m5f|1m5f]], [[1mm6|1mm6]], [[1mm7|1mm7]], [[1mqd|1mqd]], [[1mqg|1mqg]], [[1mqh|1mqh]], [[1mqi|1mqi]], [[1mqj|1mqj]], [[1ms7|1ms7]], [[1mxu|1mxu]], [[1mxv|1mxv]], [[1mxw|1mxw]], [[1mxx|1mxx]], [[1mxy|1mxy]], [[1mxz|1mxz]], [[1my0|1my0]], [[1my1|1my1]], [[1my2|1my2]], [[1my3|1my3]], [[1my4|1my4]], [[1n0t|1n0t]], [[1nnk|1nnk]], [[1nnp|1nnp]], [[1p1n|1p1n]], [[1p1o|1p1o]], [[1p1q|1p1q]], [[1p1u|1p1u]], [[1p1w|1p1w]], [[1syh|1syh]], [[1syi|1syi]], [[1wvj|1wvj]], [[1xhy|1xhy]], [[2aix|2aix]], [[2al4|2al4]], [[2al5|2al5]], [[2anj|2anj]], [[2cmo|2cmo]], [[2i3v|2i3v]], [[2i3w|2i3w]]</td></tr>
+<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2uxa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2uxa OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2uxa RCSB], [http://www.ebi.ac.uk/pdbsum/2uxa PDBsum]</span></td></tr>
+<table>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ux/2uxa_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The subunit composition determines AMPA receptor (AMPA-R) function and trafficking. Mechanisms underlying channel assembly are thus central to the efficacy and plasticity of glutamatergic synapses. We previously showed that RNA editing at the Q/R site of the GluR2 subunit contributes to the assembly of AMPA-R heteromers by attenuating formation of GluR2 homotetramers. Here we report that this function of the Q/R site depends on subunit contacts between adjacent ligand binding domains (LBDs). Changes of LBD interface contacts alter GluR2 assembly properties, forward traffic, and expression at synapses. Interestingly, developmentally regulated RNA editing within the LBD (at the R/G site) produces analogous effects. Our data reveal that editing to glycine reduces the self-assembly competence of this critical subunit and slows GluR2 maturation in the endoplasmic reticulum (ER). Therefore, RNA editing sites, located at strategic subunit interfaces, shape AMPA-R assembly and trafficking in a developmentally regulated manner.
-{{STRUCTURE_2uxa|  PDB=2uxa  |  SCENE=  }}
+Developmentally regulated, combinatorial RNA processing modulates AMPA receptor biogenesis.,Greger IH, Akamine P, Khatri L, Ziff EB Neuron. 2006 Jul 6;51(1):85-97. PMID:16815334<ref>PMID:16815334</ref>
-===CRYSTAL STRUCTURE OF THE GLUR2-FLIP LIGAND BINDING DOMAIN, R/G UNEDITED.===
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-{{ABSTRACT_PUBMED_16815334}}
-==About this Structure==
-[[2uxa]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2UXA OCA].
 ==See Also==
 *[[Ionotropic Glutamate Receptors|Ionotropic Glutamate Receptors]]
+== References ==
-==Reference==
+<references/>
-<ref group="xtra">PMID:016815334</ref><ref group="xtra">PMID:019102704</ref><references group="xtra"/>
+__TOC__
+</StructureSection>
 [[Category: Rattus norvegicus]]
 [[Category: Akamine, P.]]