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-{{Theoretical_model}}
+==THEORETICAL MODEL OF DNA-MEDIATED RNA CLEAVAGE BY A.AEOLICUS ARGONAUTE PROTEIN.==
+<StructureSection load='2ads' size='340' side='right' caption='[[2ads]]' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ADS FirstGlance]. <br>
+</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ads FirstGlance], [http://www.ebi.ac.uk/pdbsum/2ads PDBsum]</span></td></tr>
+<table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Argonaute (Ago) proteins constitute a key component of the RNA-induced silencing complex (RISC). We report the crystal structure of Aquifex aeolicus Ago (Aa-Ago) together with binding and cleavage studies, which establish this eubacterial Ago as a bona fide guide DNA strand-mediated site-specific RNA endonuclease. We have generated a stereochemically robust model of the complex, where the guide DNA-mRNA duplex is positioned within a basic channel spanning the bilobal interface, such that the 5' phosphate of the guide strand can be anchored in a basic pocket, and the mRNA can be positioned for site-specific cleavage by RNase H-type divalent cation-coordinated catalytic Asp residues of the PIWI domain. Domain swap experiments involving chimeras of human Ago (hAgo1) and cleavage-competent hAgo2 reinforce the role of the PIWI domain in "slicer" activity. We propose a four-step Ago-mediated catalytic cleavage cycle model, which provides distinct perspectives into the mechanism of guide strand-mediated mRNA cleavage within the RISC.
-[[Image:2ads.png|left|200px]]
+Crystal structure of A. aeolicus argonaute, a site-specific DNA-guided endoribonuclease, provides insights into RISC-mediated mRNA cleavage.,Yuan YR, Pei Y, Ma JB, Kuryavyi V, Zhadina M, Meister G, Chen HY, Dauter Z, Tuschl T, Patel DJ Mol Cell. 2005 Aug 5;19(3):405-19. PMID:16061186<ref>PMID:16061186</ref>
-{{STRUCTURE_2ads|  PDB=2ads  |  SCENE=  }}
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-===THEORETICAL MODEL OF DNA-MEDIATED RNA CLEAVAGE BY A.AEOLICUS ARGONAUTE PROTEIN.===
+== References ==
+<references/>
-{{ABSTRACT_PUBMED_16061186}}
+__TOC__
+</StructureSection>
-==Reference==
-<ref group="xtra">PMID:016061186</ref><references group="xtra"/>
 [[Category: Cheng, H Y]]
 [[Category: Dauter, Z]]