1g7b: Difference between revisions
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[[Image:1g7b.gif|left|200px]] | [[Image:1g7b.gif|left|200px]] | ||
'''1.3 A STRUCTURE OF T3R3 HUMAN INSULIN AT 100 K''' | {{Structure | ||
|PDB= 1g7b |SIZE=350|CAPTION= <scene name='initialview01'>1g7b</scene>, resolution 1.3Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene> and <scene name='pdbligand=ACN:ACETONE'>ACN</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''1.3 A STRUCTURE OF T3R3 HUMAN INSULIN AT 100 K''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1G7B is a [ | 1G7B is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G7B OCA]. | ||
==Reference== | ==Reference== | ||
Phase changes in T(3)R(3)(f) human insulin: temperature or pressure induced?, Smith GD, Pangborn WA, Blessing RH, Acta Crystallogr D Biol Crystallogr. 2001 Aug;57(Pt 8):1091-100. Epub 2001, Jul 23. PMID:[http:// | Phase changes in T(3)R(3)(f) human insulin: temperature or pressure induced?, Smith GD, Pangborn WA, Blessing RH, Acta Crystallogr D Biol Crystallogr. 2001 Aug;57(Pt 8):1091-100. Epub 2001, Jul 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11468392 11468392] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Blessing, R H.]] | [[Category: Blessing, R H.]] | ||
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[[Category: t3r3 human insulin hexamer]] | [[Category: t3r3 human insulin hexamer]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:19:10 2008'' | ||
Revision as of 12:19, 20 March 2008
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| , resolution 1.3Å | |||||||
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| Ligands: | , , and | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
1.3 A STRUCTURE OF T3R3 HUMAN INSULIN AT 100 K
OverviewOverview
The structure of T(3)R(3) hexameric human insulin has been determined at 100 K from two different crystals at 1.2 and 1.3 A resolution and refined to residuals of 0.169 and 0.176, respectively. Owing to a phase change, the c axis is double its room-temperature value and the asymmetric unit contains two independent TR(f) insulin dimers. Compared with the orientation in the room-temperature structure, one dimer undergoes a rotation about the c axis of -5 degrees, while the second is rotated +4 degrees. A superposition of the backbone atoms of the two independent dimers shows that the C(alpha) atoms of five residues within the R(f)-state monomers are displaced by more than 1.0 A; smaller displacements are observed for the T-state monomers. Four zinc ions lie on the crystallographic threefold axis and each forms bonds to three symmetry-related HisB10 N(varepsilon2) atoms from the T- and R(f)-state trimers. While three of the zinc ions are tetrahedrally coordinated with a chloride ion completing the coordination sphere, mixed tetrahedral/octahedral coordination is observed for one of the T-state zinc ions. The three symmetry-related "phenolic binding sites" in one hexamer contain water molecules and a glycerol molecule, but the same sites in the second hexamer are occupied by a zinc ion coordinated to an alternate conformation of HisB10, a symmetry-related HisB5 and two chloride ions. Two additional and partially occupied zinc ion sites are observed at the interface between the two independent dimers. One zinc ion is coordinated by a T-state HisB5 of one dimer, an R-state HisB5 of the second dimer and two water molecules; the second zinc ion is coordinated by an alternate side-chain conformation of the T-state HisB5 and three water molecules. The carboxyl group of one GluB13 side chain, which exists in two discrete conformations, appears to be protonated, because short contacts exist to a second carboxyl group or to a carbonyl O atom.
DiseaseDisease
Known diseases associated with this structure: Diabetes mellitus, rare form OMIM:[176730], Hyperproinsulinemia, familial OMIM:[176730], MODY, one form OMIM:[176730]
About this StructureAbout this Structure
1G7B is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
ReferenceReference
Phase changes in T(3)R(3)(f) human insulin: temperature or pressure induced?, Smith GD, Pangborn WA, Blessing RH, Acta Crystallogr D Biol Crystallogr. 2001 Aug;57(Pt 8):1091-100. Epub 2001, Jul 23. PMID:11468392
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