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[[Image:2bjc.png|left|200px]]
==NMR STRUCTURE OF A PROTEIN-DNA COMPLEX OF AN ALTERED SPECIFICITY MUTANT OF THE LAC REPRESSOR HEADPIECE THAT MIMICS THE GAL REPRESSOR==
<StructureSection load='2bjc' size='340' side='right' caption='[[2bjc]], [[NMR_Ensembles_of_Models | 16 NMR models]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2bjc]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BJC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BJC FirstGlance]. <br>
</td></tr><tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1cjg|1cjg]], [[1efa|1efa]], [[1jwl|1jwl]], [[1jye|1jye]], [[1jyf|1jyf]], [[1l1m|1l1m]], [[1lbg|1lbg]], [[1lbh|1lbh]], [[1lbi|1lbi]], [[1lcc|1lcc]], [[1lcd|1lcd]], [[1lqc|1lqc]], [[1ltp|1ltp]], [[1osl|1osl]], [[1tlf|1tlf]]</td></tr>
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bjc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bjc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2bjc RCSB], [http://www.ebi.ac.uk/pdbsum/2bjc PDBsum]</span></td></tr>
<table>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bj/2bjc_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Recognition of the lac operator by the lac repressor involves specific interactions between residues in the repressor's recognition helix and bases in the DNA major groove. Tyr17 and Gln18, at positions 1 and 2 in the lac repressor recognition helix, can be exchanged for other amino acids to generate mutant repressors that display altered specificity. We have solved the solution structure of a protein-DNA complex of an altered-specificity mutant lac headpiece in which Tyr17 and Gln18 were exchanged for valine and alanine, respectively, as found in the recognition helix of the gal repressor. As previously described by Lehming et al. (EMBO J. 1987, 6, 3145-3153), this altered-specificity mutant of the lac repressor recognizes a variant lac operator that is similar to the gal operator Oe. The mutant lac headpiece showed the predicted specificity and is also able to mimic the gal repressor by recognizing and bending the natural gal operator Oe. These structural data show that, while most of the anchoring points that help the lac headpiece to assemble on the lac operator were preserved, a different network of protein-DNA interactions connecting Ala17 and Val18 to bases in the DNA major groove drives the specificity towards the altered operator.


{{STRUCTURE_2bjc|  PDB=2bjc  |  SCENE=  }}
Altered specificity in DNA binding by the lac repressor: a mutant lac headpiece that mimics the gal repressor.,Kopke Salinas R, Folkers GE, Bonvin AM, Das D, Boelens R, Kaptein R Chembiochem. 2005 Sep;6(9):1628-37. PMID:16094693<ref>PMID:16094693</ref>


===NMR STRUCTURE OF A PROTEIN-DNA COMPLEX OF AN ALTERED SPECIFICITY MUTANT OF THE LAC REPRESSOR HEADPIECE THAT MIMICS THE GAL REPRESSOR===
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 
</div>
{{ABSTRACT_PUBMED_16094693}}
 
==About this Structure==
[[2bjc]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BJC OCA].


==See Also==
==See Also==
*[[Lac repressor|Lac repressor]]
*[[Lac repressor 3D structures|Lac repressor 3D structures]]
 
== References ==
==Reference==
<references/>
<ref group="xtra">PMID:016094693</ref><ref group="xtra">PMID:012065400</ref><ref group="xtra">PMID:002826131</ref><references group="xtra"/>
__TOC__
</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Boelens, R.]]
[[Category: Boelens, R.]]

Revision as of 08:27, 29 September 2014

NMR STRUCTURE OF A PROTEIN-DNA COMPLEX OF AN ALTERED SPECIFICITY MUTANT OF THE LAC REPRESSOR HEADPIECE THAT MIMICS THE GAL REPRESSORNMR STRUCTURE OF A PROTEIN-DNA COMPLEX OF AN ALTERED SPECIFICITY MUTANT OF THE LAC REPRESSOR HEADPIECE THAT MIMICS THE GAL REPRESSOR

Structural highlights

2bjc is a 4 chain structure with sequence from Escherichia coli. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:1cjg, 1efa, 1jwl, 1jye, 1jyf, 1l1m, 1lbg, 1lbh, 1lbi, 1lcc, 1lcd, 1lqc, 1ltp, 1osl, 1tlf
Resources:FirstGlance, OCA, RCSB, PDBsum

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Recognition of the lac operator by the lac repressor involves specific interactions between residues in the repressor's recognition helix and bases in the DNA major groove. Tyr17 and Gln18, at positions 1 and 2 in the lac repressor recognition helix, can be exchanged for other amino acids to generate mutant repressors that display altered specificity. We have solved the solution structure of a protein-DNA complex of an altered-specificity mutant lac headpiece in which Tyr17 and Gln18 were exchanged for valine and alanine, respectively, as found in the recognition helix of the gal repressor. As previously described by Lehming et al. (EMBO J. 1987, 6, 3145-3153), this altered-specificity mutant of the lac repressor recognizes a variant lac operator that is similar to the gal operator Oe. The mutant lac headpiece showed the predicted specificity and is also able to mimic the gal repressor by recognizing and bending the natural gal operator Oe. These structural data show that, while most of the anchoring points that help the lac headpiece to assemble on the lac operator were preserved, a different network of protein-DNA interactions connecting Ala17 and Val18 to bases in the DNA major groove drives the specificity towards the altered operator.

Altered specificity in DNA binding by the lac repressor: a mutant lac headpiece that mimics the gal repressor.,Kopke Salinas R, Folkers GE, Bonvin AM, Das D, Boelens R, Kaptein R Chembiochem. 2005 Sep;6(9):1628-37. PMID:16094693[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

See Also

References

  1. ↑ Kopke Salinas R, Folkers GE, Bonvin AM, Das D, Boelens R, Kaptein R. Altered specificity in DNA binding by the lac repressor: a mutant lac headpiece that mimics the gal repressor. Chembiochem. 2005 Sep;6(9):1628-37. PMID:16094693 doi:10.1002/cbic.200500049
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