1g59: Difference between revisions

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Revision as of 12:18, 20 March 2008

File:1g59.gif

, resolution 2.4Å

Activity:

Glutamate--tRNA ligase, with EC number 6.1.1.17

Coordinates:

save as pdb, mmCIF, xml

GLUTAMYL-TRNA SYNTHETASE COMPLEXED WITH TRNA(GLU).

OverviewOverview

Glutamyl-tRNA synthetases (GluRSs) are divided into two distinct types, with regard to the presence or absence of glutaminyl-tRNA synthetase (GlnRS) in the genetic translation systems. In the original 19-synthetase systems lacking GlnRS, the 'non-discriminating' GluRS glutamylates both tRNAGlu and tRNAGln. In contrast, in the evolved 20-synthetase systems with GlnRS, the 'discriminating' GluRS aminoacylates only tRNAGlu. Here we report the 2.4 A resolution crystal structure of a 'discriminating' GluRS.tRNAGlu complex from Thermus thermophilus. The GluRS recognizes the tRNAGlu anticodon bases via two alpha-helical domains, maintaining the base stacking. We show that the discrimination between the Glu and Gln anticodons (34YUC36 and 34YUG36, respectively) is achieved by a single arginine residue (Arg 358). The mutation of Arg 358 to Gln resulted in a GluRS that does not discriminate between the Glu and Gln anticodons. This change mimics the reverse course of GluRS evolution from anticodon 'non-dicsriminating' to 'discriminating'.

About this StructureAbout this Structure

1G59 is a Single protein structure of sequence from Thermus thermophilus. Full crystallographic information is available from OCA.

ReferenceReference

Structural basis for anticodon recognition by discriminating glutamyl-tRNA synthetase., Sekine S, Nureki O, Shimada A, Vassylyev DG, Yokoyama S, Nat Struct Biol. 2001 Mar;8(3):203-6. PMID:11224561

Page seeded by OCA on Thu Mar 20 11:18:21 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1g59.gif|left|200px]]<br /><applet load="1g59" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1g59.gif|left|200px]]
-caption="1g59, resolution 2.4&Aring;" />
-'''GLUTAMYL-TRNA SYNTHETASE COMPLEXED WITH TRNA(GLU).'''<br />
+ {{Structure
+|PDB= 1g59 |SIZE=350|CAPTION= <scene name='initialview01'>1g59</scene>, resolution 2.4&Aring;
+|SITE=
+|LIGAND=
+|ACTIVITY= [http://en.wikipedia.org/wiki/Glutamate--tRNA_ligase Glutamate--tRNA ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.17 6.1.1.17]
+|GENE=
+}}
+'''GLUTAMYL-TRNA SYNTHETASE COMPLEXED WITH TRNA(GLU).'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-G59 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Active as [http://en.wikipedia.org/wiki/Glutamate--tRNA_ligase Glutamate--tRNA ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.17 6.1.1.17] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G59 OCA].
+G59 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G59 OCA].
 ==Reference==
-Structural basis for anticodon recognition by discriminating glutamyl-tRNA synthetase., Sekine S, Nureki O, Shimada A, Vassylyev DG, Yokoyama S, Nat Struct Biol. 2001 Mar;8(3):203-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11224561 11224561]
+Structural basis for anticodon recognition by discriminating glutamyl-tRNA synthetase., Sekine S, Nureki O, Shimada A, Vassylyev DG, Yokoyama S, Nat Struct Biol. 2001 Mar;8(3):203-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11224561 11224561]
 [[Category: Glutamate--tRNA ligase]]
 [[Category: Single protein]]
@@ Line 24: / Line 33: @@
 [[Category: riken structural genomics/proteomics initiative]]
 [[Category: rsgi]]
-[[Category: structural genomics]]
+[[Category: structural genomic]]
 [[Category: transfer rna]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:46:13 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:18:21 2008''