1g0t: Difference between revisions

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Revision as of 12:16, 20 March 2008

File:1g0t.jpg

, resolution 2.60Å

Ligands:

Activity:

Protein disulfide-isomerase, with EC number 5.3.4.1

Coordinates:

save as pdb, mmCIF, xml

DSBC MUTANT C101S

OverviewOverview

DsbC is one of five Escherichia coli proteins required for disulfide bond formation and is thought to function as a disulfide bond isomerase during oxidative protein folding in the periplasm. DsbC is a 2 x 23 kDa homodimer and has both protein disulfide isomerase and chaperone activity. We report the 1.9 A resolution crystal structure of oxidized DsbC where both Cys-X-X-Cys active sites form disulfide bonds. The molecule consists of separate thioredoxin-like domains joined via hinged linker helices to an N-terminal dimerization domain. The hinges allow relative movement of the active sites, and a broad uncharged cleft between them may be involved in peptide binding and DsbC foldase activities.

About this StructureAbout this Structure

1G0T is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure of the protein disulfide bond isomerase, DsbC, from Escherichia coli., McCarthy AA, Haebel PW, Torronen A, Rybin V, Baker EN, Metcalf P, Nat Struct Biol. 2000 Mar;7(3):196-9. PMID:10700276

Page seeded by OCA on Thu Mar 20 11:16:36 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1g0t.jpg|left|200px]]<br /><applet load="1g0t" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1g0t.jpg|left|200px]]
-caption="1g0t, resolution 2.60&Aring;" />
-'''DSBC MUTANT C101S'''<br />
+ {{Structure
+|PDB= 1g0t |SIZE=350|CAPTION= <scene name='initialview01'>1g0t</scene>, resolution 2.60&Aring;
+|SITE=
+|LIGAND= <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>
+|ACTIVITY= [http://en.wikipedia.org/wiki/Protein_disulfide-isomerase Protein disulfide-isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.4.1 5.3.4.1]
+|GENE=
+}}
+'''DSBC MUTANT C101S'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-G0T is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=PEG:'>PEG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Protein_disulfide-isomerase Protein disulfide-isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.4.1 5.3.4.1] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G0T OCA].
+G0T is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G0T OCA].
 ==Reference==
-Crystal structure of the protein disulfide bond isomerase, DsbC, from Escherichia coli., McCarthy AA, Haebel PW, Torronen A, Rybin V, Baker EN, Metcalf P, Nat Struct Biol. 2000 Mar;7(3):196-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10700276 10700276]
+Crystal structure of the protein disulfide bond isomerase, DsbC, from Escherichia coli., McCarthy AA, Haebel PW, Torronen A, Rybin V, Baker EN, Metcalf P, Nat Struct Biol. 2000 Mar;7(3):196-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10700276 10700276]
 [[Category: Escherichia coli]]
 [[Category: Protein disulfide-isomerase]]
@@ Line 21: / Line 30: @@
 [[Category: thioredoxin fold]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:44:51 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:16:36 2008''