1fl8: Difference between revisions

Revision as of 12:10, 20 March 2008

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HYPERMODIFIED NUCLEOSIDES IN THE ANTICODON OF TRNALYS STABILIZE A CANONICAL U-TURN STRUCTURE

OverviewOverview

Modified nucleosides in the anticodon domain of Escherichia coli tRNA(Lys) are necessary for high-affinity codon recognition and reading frame maintenance. Human tRNA(Lys,3) is the specific primer for HIV-1 reverse transcriptase and also requires nucleoside modification for proper function. We now present NMR solution structures for the fully modified 17-nucleotide E. coli tRNA(Lys) anticodon stem-loop domain (ASL). NMR data were also collected for several partially modified ASLs, revealing the contributions each modified nucleoside (mnm(5)s(2)U34, t(6)A37, and psi39) makes in transforming the disordered, unmodified tRNA ASL into the highly ordered native structure. The solution structure of the native ASL domain provides insight into longstanding questions regarding both wobble position modification and the nearly ubiquitous t(6)A37 found in tRNAs with an adjacent U at position 36. Native tRNA(Lys) has a U-turn structure similar to the yeast tRNA(Phe) crystal structure, unlike previously proposed "unconventional" anticodon structures characterized by stable interactions between mnm(5)s(2)U-34 and t(6)A-37.

About this StructureAbout this Structure

1FL8 is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.

ReferenceReference

Hypermodified nucleosides in the anticodon of tRNALys stabilize a canonical U-turn structure., Sundaram M, Durant PC, Davis DR, Biochemistry. 2000 Oct 17;39(41):12575-84. PMID:11027137

Page seeded by OCA on Thu Mar 20 11:10:32 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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-[[Image:1fl8.gif|left|200px]]<br /><applet load="1fl8" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1fl8.gif|left|200px]]
-caption="1fl8" />
-'''HYPERMODIFIED NUCLEOSIDES IN THE ANTICODON OF TRNALYS STABILIZE A CANONICAL U-TURN STRUCTURE'''<br />
+ {{Structure
+|PDB= 1fl8 |SIZE=350|CAPTION= <scene name='initialview01'>1fl8</scene>
+|SITE=
+|LIGAND=
+|ACTIVITY=
+|GENE=
+}}
+'''HYPERMODIFIED NUCLEOSIDES IN THE ANTICODON OF TRNALYS STABILIZE A CANONICAL U-TURN STRUCTURE'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-FL8 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FL8 OCA].
+FL8 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FL8 OCA].
 ==Reference==
-Hypermodified nucleosides in the anticodon of tRNALys stabilize a canonical U-turn structure., Sundaram M, Durant PC, Davis DR, Biochemistry. 2000 Oct 17;39(41):12575-84. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11027137 11027137]
+Hypermodified nucleosides in the anticodon of tRNALys stabilize a canonical U-turn structure., Sundaram M, Durant PC, Davis DR, Biochemistry. 2000 Oct 17;39(41):12575-84. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11027137 11027137]
 [[Category: Protein complex]]
 [[Category: Davis, D R.]]
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 [[Category: trna]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:39:49 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:10:32 2008''