1fl3: Difference between revisions
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'''CRYSTAL STRUCTURE OF THE BLUE FLUORESCENT ANTIBODY (19G2) IN COMPLEX WITH STILBENE HAPTEN AT 277K''' | {{Structure | ||
|PDB= 1fl3 |SIZE=350|CAPTION= <scene name='initialview01'>1fl3</scene>, resolution 2.45Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=SPB:4-(4-STYRYL-PHENYLCARBAMOYL)-BUTYRIC ACID'>SPB</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''CRYSTAL STRUCTURE OF THE BLUE FLUORESCENT ANTIBODY (19G2) IN COMPLEX WITH STILBENE HAPTEN AT 277K''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1FL3 is a [ | 1FL3 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FL3 OCA]. | ||
==Reference== | ==Reference== | ||
Blue-fluorescent antibodies., Simeonov A, Matsushita M, Juban EA, Thompson EH, Hoffman TZ, Beuscher AE 4th, Taylor MJ, Wirsching P, Rettig W, McCusker JK, Stevens RC, Millar DP, Schultz PG, Lerner RA, Janda KD, Science. 2000 Oct 13;290(5490):307-13. PMID:[http:// | Blue-fluorescent antibodies., Simeonov A, Matsushita M, Juban EA, Thompson EH, Hoffman TZ, Beuscher AE 4th, Taylor MJ, Wirsching P, Rettig W, McCusker JK, Stevens RC, Millar DP, Schultz PG, Lerner RA, Janda KD, Science. 2000 Oct 13;290(5490):307-13. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11030644 11030644] | ||
[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: immunoglobulin fold]] | [[Category: immunoglobulin fold]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:10:31 2008'' | ||
Revision as of 12:10, 20 March 2008
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF THE BLUE FLUORESCENT ANTIBODY (19G2) IN COMPLEX WITH STILBENE HAPTEN AT 277K
OverviewOverview
The forte of catalytic antibodies has resided in the control of the ground-state reaction coordinate. A principle and method are now described in which antibodies can direct the outcome of photophysical and photochemical events that take place on excited-state potential energy surfaces. The key component is a chemically reactive optical sensor that provides a direct report of the dynamic interplay between protein and ligand at the active site. To illustrate the concept, we used a trans-stilbene hapten to elicit a panel of monoclonal antibodies that displayed a range of fluorescent spectral behavior when bound to a trans-stilbene substrate. Several antibodies yielded a blue fluorescence indicative of an excited-state complex or "exciplex" between trans-stilbene and the antibody. The antibodies controlled the isomerization coordinate of trans-stilbene and dynamically coupled this manifold with an active-site residue. A step was taken toward the use of antibody-based photochemical sensors for diagnostic and clinical applications.
About this StructureAbout this Structure
1FL3 is a Protein complex structure of sequences from Mus musculus. Full crystallographic information is available from OCA.
ReferenceReference
Blue-fluorescent antibodies., Simeonov A, Matsushita M, Juban EA, Thompson EH, Hoffman TZ, Beuscher AE 4th, Taylor MJ, Wirsching P, Rettig W, McCusker JK, Stevens RC, Millar DP, Schultz PG, Lerner RA, Janda KD, Science. 2000 Oct 13;290(5490):307-13. PMID:11030644
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