1fkv: Difference between revisions
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[[Image:1fkv.gif|left|200px]] | [[Image:1fkv.gif|left|200px]] | ||
'''RECOMBINANT GOAT ALPHA-LACTALBUMIN T29I''' | {{Structure | ||
|PDB= 1fkv |SIZE=350|CAPTION= <scene name='initialview01'>1fkv</scene>, resolution 2.0Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=CA:CALCIUM ION'>CA</scene> | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/Lactose_synthase Lactose synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.22 2.4.1.22] | |||
|GENE= A CLONED GENE OF GOAT LACTA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9925 Capra hircus]) | |||
}} | |||
'''RECOMBINANT GOAT ALPHA-LACTALBUMIN T29I''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1FKV is a [ | 1FKV is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Capra_hircus Capra hircus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FKV OCA]. | ||
==Reference== | ==Reference== | ||
Contribution of Thr29 to the thermodynamic stability of goat alpha-lactalbumin as determined by experimental and theoretical approaches., Horii K, Saito M, Yoda T, Tsumoto K, Matsushima M, Kuwajima K, Kumagai I, Proteins. 2001 Oct 1;45(1):16-29. PMID:[http:// | Contribution of Thr29 to the thermodynamic stability of goat alpha-lactalbumin as determined by experimental and theoretical approaches., Horii K, Saito M, Yoda T, Tsumoto K, Matsushima M, Kuwajima K, Kumagai I, Proteins. 2001 Oct 1;45(1):16-29. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11536356 11536356] | ||
[[Category: Capra hircus]] | [[Category: Capra hircus]] | ||
[[Category: Lactose synthase]] | [[Category: Lactose synthase]] | ||
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[[Category: lactose synthase component]] | [[Category: lactose synthase component]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:10:24 2008'' | ||
Revision as of 12:10, 20 March 2008
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| , resolution 2.0Å | |||||||
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| Ligands: | |||||||
| Gene: | A CLONED GENE OF GOAT LACTA (Capra hircus) | ||||||
| Activity: | Lactose synthase, with EC number 2.4.1.22 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
RECOMBINANT GOAT ALPHA-LACTALBUMIN T29I
OverviewOverview
The Thr29 residue in the hydrophobic core of goat alpha-lactalbumin (alpha-LA) was substituted with Val (Thr29Val) and Ile (Thr29Ile) to investigate the contribution of Thr29 to the thermodynamic stability of the protein. We carried out protein stability measurements, X-ray crystallographic analyses, and free energy calculations based on molecular dynamics simulation. The equilibrium unfolding transitions induced by guanidine hydrochloride demonstrated that the Thr29Val and Thr29Ile mutants were, respectively, 1.9 and 3.2 kcal/mol more stable than the wild-type protein (WT). The overall structures of the mutants were almost identical to that of WT, in spite of the disruption of the hydrogen bonding between the side-chain O-H group of Thr29 and the main-chain C=O group of Glu25. To analyze the stabilization mechanism of the mutants, we performed free energy calculations. The calculated free energy differences were in good agreement with the experimental values. The stabilization of the mutants was mainly caused by solvation loss in the denatured state. Furthermore, the O-H group of Thr29 favorably interacts with the C=O group of Glu25 to form hydrogen bonds and, simultaneously, unfavorably interacts electrostatically with the main-chain C=O group of Thr29. The difference in the free energy profile of the unfolding path between WT and the Thr29Ile mutant is discussed in light of our experimental and theoretical results.
About this StructureAbout this Structure
1FKV is a Single protein structure of sequence from Capra hircus. Full crystallographic information is available from OCA.
ReferenceReference
Contribution of Thr29 to the thermodynamic stability of goat alpha-lactalbumin as determined by experimental and theoretical approaches., Horii K, Saito M, Yoda T, Tsumoto K, Matsushima M, Kuwajima K, Kumagai I, Proteins. 2001 Oct 1;45(1):16-29. PMID:11536356
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