1fhy: Difference between revisions

← Older edit Newer edit →

Revision as of 12:09, 20 March 2008

File:1fhy.gif

, resolution 2.20Å

Ligands:

and

Coordinates:

save as pdb, mmCIF, xml

PSORALEN CROSS-LINKED D(CCGCTAGCGG) FORMS HOLLIDAY JUNCTION

OverviewOverview

The single-crystal structures are presented for two DNA sequences with the thymine bases covalently cross-linked across the complementary strands by 4'-hydroxymethyl-4,5',8-trimethylpsoralen (HMT). The HMT-adduct of d(CCGCTAGCGG) forms a psoralen-induced Holliday junction, showing for the first time the effect of this important class of chemotheraputics on the structure of the recombination intermediate. In contrast, HMT-d(CCGGTACCGG) forms a sequence-dependent junction. In both structures, the DNA duplex is highly distorted at the thymine base linked to the six-member pyrone ring of the drug. The psoralen cross-link defines the intramolecular interactions of the drug-induced junction, while the sequence-dependent structure is nearly identical to the native Holliday junction of d(CCGGTACCGG) alone. The two structures contrast the effects of drug- and sequence-dependent interactions on the structure of a Holliday junction, suggesting a role for psoralen in the mechanism to initiate repair of psoralen-lesions in mammalian DNA.

About this StructureAbout this Structure

1FHY is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.

ReferenceReference

The crystal structures of psoralen cross-linked DNAs: drug-dependent formation of Holliday junctions., Eichman BF, Mooers BH, Alberti M, Hearst JE, Ho PS, J Mol Biol. 2001 Apr 20;308(1):15-26. PMID:11302703

Page seeded by OCA on Thu Mar 20 11:09:21 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1fhy.gif|left|200px]]<br /><applet load="1fhy" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1fhy.gif|left|200px]]
-caption="1fhy, resolution 2.20&Aring;" />
-'''PSORALEN CROSS-LINKED D(CCGCTAGCGG) FORMS HOLLIDAY JUNCTION'''<br />
+ {{Structure
+|PDB= 1fhy |SIZE=350|CAPTION= <scene name='initialview01'>1fhy</scene>, resolution 2.20&Aring;
+|SITE=
+|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene> and <scene name='pdbligand=PSO:4'-HYDROXYMETHYL-4,5',8-TRIMETHYLPSORALEN'>PSO</scene>
+|ACTIVITY=
+|GENE=
+}}
+'''PSORALEN CROSS-LINKED D(CCGCTAGCGG) FORMS HOLLIDAY JUNCTION'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-FHY is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=PSO:'>PSO</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FHY OCA].
+FHY is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FHY OCA].
 ==Reference==
-The crystal structures of psoralen cross-linked DNAs: drug-dependent formation of Holliday junctions., Eichman BF, Mooers BH, Alberti M, Hearst JE, Ho PS, J Mol Biol. 2001 Apr 20;308(1):15-26. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11302703 11302703]
+The crystal structures of psoralen cross-linked DNAs: drug-dependent formation of Holliday junctions., Eichman BF, Mooers BH, Alberti M, Hearst JE, Ho PS, J Mol Biol. 2001 Apr 20;308(1):15-26. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11302703 11302703]
 [[Category: Protein complex]]
 [[Category: Alberti, M.]]
@@ Line 25: / Line 34: @@
 [[Category: psoralen]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:38:50 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:09:21 2008''