1fh1: Difference between revisions
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[[Image:1fh1.gif|left|200px]] | [[Image:1fh1.gif|left|200px]] | ||
'''BACKBONE FOLD OF NODF''' | {{Structure | ||
|PDB= 1fh1 |SIZE=350|CAPTION= <scene name='initialview01'>1fh1</scene> | |||
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|LIGAND= | |||
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'''BACKBONE FOLD OF NODF''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1FH1 is a [ | 1FH1 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rhizobium_leguminosarum Rhizobium leguminosarum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FH1 OCA]. | ||
==Reference== | ==Reference== | ||
Rapid determination of protein folds using residual dipolar couplings., Fowler CA, Tian F, Al-Hashimi HM, Prestegard JH, J Mol Biol. 2000 Dec 1;304(3):447-60. PMID:[http:// | Rapid determination of protein folds using residual dipolar couplings., Fowler CA, Tian F, Al-Hashimi HM, Prestegard JH, J Mol Biol. 2000 Dec 1;304(3):447-60. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11090286 11090286] | ||
[[Category: Rhizobium leguminosarum]] | [[Category: Rhizobium leguminosarum]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: root nodulation factor]] | [[Category: root nodulation factor]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:08:58 2008'' | ||
Revision as of 12:09, 20 March 2008
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BACKBONE FOLD OF NODF
OverviewOverview
Over the next few years, various genome projects will sequence many new genes and yield many new gene products. Many of these products will have no known function and little, if any, sequence homology to existing proteins. There is reason to believe that a rapid determination of a protein fold, even at low resolution, can aid in the identification of function and expedite the determination of structure at higher resolution. Recently devised NMR methods of measuring residual dipolar couplings provide one route to the determination of a fold. They do this by allowing the alignment of previously identified secondary structural elements with respect to each other. When combined with constraints involving loops connecting elements or other short-range experimental distance information, a fold is produced. We illustrate this approach to protein fold determination on (15)N-labeled Eschericia coli acyl carrier protein using a limited set of (15)N-(1)H and (1)H-(1)H dipolar couplings. We also illustrate an approach using a more extended set of heteronuclear couplings on a related protein, (13)C, (15)N-labeled NodF protein from Rhizobium leguminosarum.
About this StructureAbout this Structure
1FH1 is a Single protein structure of sequence from Rhizobium leguminosarum. Full crystallographic information is available from OCA.
ReferenceReference
Rapid determination of protein folds using residual dipolar couplings., Fowler CA, Tian F, Al-Hashimi HM, Prestegard JH, J Mol Biol. 2000 Dec 1;304(3):447-60. PMID:11090286
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