1fca: Difference between revisions
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[[Image:1fca.jpg|left|200px]] | [[Image:1fca.jpg|left|200px]] | ||
'''STRUCTURE OF THE FERREDOXIN FROM CLOSTRIDIUM ACIDURICI: MODEL AT 1.8 ANGSTROMS RESOLUTION''' | {{Structure | ||
|PDB= 1fca |SIZE=350|CAPTION= <scene name='initialview01'>1fca</scene>, resolution 1.8Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=SF4:IRON/SULFUR CLUSTER'>SF4</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''STRUCTURE OF THE FERREDOXIN FROM CLOSTRIDIUM ACIDURICI: MODEL AT 1.8 ANGSTROMS RESOLUTION''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1FCA is a [ | 1FCA is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Clostridium_acidurici Clostridium acidurici]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FCA OCA]. | ||
==Reference== | ==Reference== | ||
Structure of the ferredoxin from Clostridium acidurici: model at 1.8 A resolution., TranQui D, Jesior JC, Acta Crystallogr D Biol Crystallogr. 1995 Mar 1;51(Pt 2):155-9. PMID:[http:// | Structure of the ferredoxin from Clostridium acidurici: model at 1.8 A resolution., TranQui D, Jesior JC, Acta Crystallogr D Biol Crystallogr. 1995 Mar 1;51(Pt 2):155-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15299316 15299316] | ||
[[Category: Clostridium acidurici]] | [[Category: Clostridium acidurici]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: electron transport (cytochrome)]] | [[Category: electron transport (cytochrome)]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:07:16 2008'' | ||
Revision as of 12:07, 20 March 2008
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
STRUCTURE OF THE FERREDOXIN FROM CLOSTRIDIUM ACIDURICI: MODEL AT 1.8 ANGSTROMS RESOLUTION
OverviewOverview
Ferredoxins (Fd) are electron-carrier proteins, the active sites of which are organized around clusters made of iron and inorganic sulfur. The Fd from Clostridium acidurici is 55 amino acids long and contains two [4Fe-4S] clusters. Crystals have been obtained in the space group P4(3)2(1)2, a = b = 34.441 (5), c = 74.778 (9) A. The structure was solved by molecular replacement using the Fd from Peptostreptcoccus asaccharolyticus as a search model, these two ferredoxins having 37 residues in common. Refinement using molecular-dynamics techniques was then initiated. Successive rounds of model building and refinement gave a structure that includes 45 water molecules with R = 15%. At this stage, the electron-density map clearly revealed discrepancies in the position of two amino acids in the published primary sequence. Refinement based on these modifications led to R = 14.3% for 3921 reflections up to 1.8 A, resolution. The geometry of the two clusters has been found to be in good agreement with that previously obtained at a lower resolution. Interactions of polypeptide chain with the [4Fe-4S] clusters, the cluster geometry as well as the hydrogen bonds involving S, Sgamma, N and water molecules are reported.
About this StructureAbout this Structure
1FCA is a Single protein structure of sequence from Clostridium acidurici. Full crystallographic information is available from OCA.
ReferenceReference
Structure of the ferredoxin from Clostridium acidurici: model at 1.8 A resolution., TranQui D, Jesior JC, Acta Crystallogr D Biol Crystallogr. 1995 Mar 1;51(Pt 2):155-9. PMID:15299316
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