1f89: Difference between revisions

Revision as of 12:05, 20 March 2008

, resolution 2.4Å

Coordinates:

Crystal structure of Saccharomyces cerevisiae Nit3, a member of branch 10 of the nitrilase superfamily

OverviewOverview

The crystal structure of a yeast hypothetical protein with sequence similarity to CN hydrolases has been determined to 2.4 A resolution by the multiwavelength anomalous dispersion (MAD) method. The protein folds as a four-layer alphabetabetaalpha sandwich and exists as a dimer in the crystal and in solution. It was selected in a structural genomics project as representative of CN hydrolases at a time when no structures had been determined for members of this family. Structures for two other members of the family have since been reported and the three proteins have similar topology and dimerization modes, which are distinct from those of other alphabetabetaalpha proteins whose structures are known. The dimers form an unusual eight-layer alphabetabetaalpha:alphabetabetaalpha structure. Although the precise enzymatic reactions catalyzed by the yeast protein are not known, considerable information about the active site may be deduced from conserved sequence motifs, comparative biochemical information, and comparison with known structures of hydrolase active sites. As with serine hydrolases, the active-site nucleophile (cysteine in this case) is positioned on a nucleophile elbow.

About this StructureAbout this Structure

1F89 is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure of a putative CN hydrolase from yeast., Kumaran D, Eswaramoorthy S, Gerchman SE, Kycia H, Studier FW, Swaminathan S, Proteins. 2003 Aug 1;52(2):283-91. PMID:12833551

Page seeded by OCA on Thu Mar 20 11:05:48 2008

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OCA

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-[[Image:1f89.gif|left|200px]]<br /><applet load="1f89" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1f89.gif|left|200px]]
-caption="1f89, resolution 2.4&Aring;" />
-'''Crystal structure of Saccharomyces cerevisiae Nit3, a member of branch 10 of the nitrilase superfamily'''<br />
+ {{Structure
+|PDB= 1f89 |SIZE=350|CAPTION= <scene name='initialview01'>1f89</scene>, resolution 2.4&Aring;
+|SITE=
+|LIGAND=
+|ACTIVITY=
+|GENE=
+}}
+'''Crystal structure of Saccharomyces cerevisiae Nit3, a member of branch 10 of the nitrilase superfamily'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-F89 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F89 OCA].
+F89 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F89 OCA].
 ==Reference==
-Crystal structure of a putative CN hydrolase from yeast., Kumaran D, Eswaramoorthy S, Gerchman SE, Kycia H, Studier FW, Swaminathan S, Proteins. 2003 Aug 1;52(2):283-91. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12833551 12833551]
+Crystal structure of a putative CN hydrolase from yeast., Kumaran D, Eswaramoorthy S, Gerchman SE, Kycia H, Studier FW, Swaminathan S, Proteins. 2003 Aug 1;52(2):283-91. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12833551 12833551]
 [[Category: Saccharomyces cerevisiae]]
 [[Category: Single protein]]
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 [[Category: protein structure initiative]]
 [[Category: psi]]
-[[Category: structural genomics]]
+[[Category: structural genomic]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:35:55 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:05:48 2008''