1f5q: Difference between revisions

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Revision as of 12:04, 20 March 2008

File:1f5q.gif

, resolution 2.50Å

Ligands:

Coordinates:

save as pdb, mmCIF, xml

CRYSTAL STRUCTURE OF MURINE GAMMA HERPESVIRUS CYCLIN COMPLEXED TO HUMAN CYCLIN DEPENDENT KINASE 2

OverviewOverview

Several gamma-herpesviruses encode proteins related to the mammalian cyclins, regulatory subunits of cyclin-dependent kinases (cdks) essential for cell cycle progression. We report a 2.5 A crystal structure of a full-length oncogenic viral cyclin from gamma-herpesvirus 68 complexed with cdk2. The viral cyclin binds cdk2 with an orientation different from cyclin A and makes several novel interactions at the interface, yet it activates cdk2 by triggering conformational changes similar to cyclin A. Sequences within the viral cyclin N-terminus lock part of the cdk2 T-loop within the core of the complex. These sequences and others are conserved amongst the viral and cellular D-type cyclins, suggesting that this structure has wider implications for other cyclin-cdk complexes. The observed resistance of this viral cyclin-cdk complex to inhibition by the p27(KIP:) cdk inhibitor is explained by sequence and conformational variation in the cyclin rendering the p27(KIP:)-binding site on the cyclin subunit non-functional.

About this StructureAbout this Structure

1F5Q is a Protein complex structure of sequences from Homo sapiens and Murid herpesvirus 1. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure of a gamma-herpesvirus cyclin-cdk complex., Card GL, Knowles P, Laman H, Jones N, McDonald NQ, EMBO J. 2000 Jun 15;19(12):2877-88. PMID:10856233

Page seeded by OCA on Thu Mar 20 11:04:50 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1f5q.gif|left|200px]]<br /><applet load="1f5q" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1f5q.gif|left|200px]]
-caption="1f5q, resolution 2.50&Aring;" />
-'''CRYSTAL STRUCTURE OF MURINE GAMMA HERPESVIRUS CYCLIN COMPLEXED TO HUMAN CYCLIN DEPENDENT KINASE 2'''<br />
+ {{Structure
+|PDB= 1f5q |SIZE=350|CAPTION= <scene name='initialview01'>1f5q</scene>, resolution 2.50&Aring;
+|SITE=
+|LIGAND= <scene name='pdbligand=CL:CHLORIDE ION'>CL</scene>
+|ACTIVITY=
+|GENE=
+}}
+'''CRYSTAL STRUCTURE OF MURINE GAMMA HERPESVIRUS CYCLIN COMPLEXED TO HUMAN CYCLIN DEPENDENT KINASE 2'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-F5Q is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Murid_herpesvirus_1 Murid herpesvirus 1] with <scene name='pdbligand=CL:'>CL</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F5Q OCA].
+F5Q is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Murid_herpesvirus_1 Murid herpesvirus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F5Q OCA].
 ==Reference==
-Crystal structure of a gamma-herpesvirus cyclin-cdk complex., Card GL, Knowles P, Laman H, Jones N, McDonald NQ, EMBO J. 2000 Jun 15;19(12):2877-88. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10856233 10856233]
+Crystal structure of a gamma-herpesvirus cyclin-cdk complex., Card GL, Knowles P, Laman H, Jones N, McDonald NQ, EMBO J. 2000 Jun 15;19(12):2877-88. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10856233 10856233]
 [[Category: Homo sapiens]]
 [[Category: Murid herpesvirus 1]]
@@ Line 23: / Line 32: @@
 [[Category: herpesviral cyclin]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:35:30 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:04:50 2008''