1f4o: Difference between revisions
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'''CRYSTAL STRUCTURE OF GRANCALCIN WITH BOUND CALCIUM''' | {{Structure | ||
|PDB= 1f4o |SIZE=350|CAPTION= <scene name='initialview01'>1f4o</scene>, resolution 2.5Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=CA:CALCIUM ION'>CA</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''CRYSTAL STRUCTURE OF GRANCALCIN WITH BOUND CALCIUM''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1F4O is a [ | 1F4O is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F4O OCA]. | ||
==Reference== | ==Reference== | ||
Crystal structure of human grancalcin, a member of the penta-EF-hand protein family., Jia J, Han Q, Borregaard N, Lollike K, Cygler M, J Mol Biol. 2000 Jul 28;300(5):1271-81. PMID:[http:// | Crystal structure of human grancalcin, a member of the penta-EF-hand protein family., Jia J, Han Q, Borregaard N, Lollike K, Cygler M, J Mol Biol. 2000 Jul 28;300(5):1271-81. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10903868 10903868] | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: penta-ef-hand protein]] | [[Category: penta-ef-hand protein]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:04:23 2008'' | ||
Revision as of 12:04, 20 March 2008
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CRYSTAL STRUCTURE OF GRANCALCIN WITH BOUND CALCIUM
OverviewOverview
Grancalcin is a Ca(2+)-binding protein expressed at high level in neutrophils. It belongs to the PEF family, proteins containing five EF-hand motifs and which are known to associate with membranes in Ca(2+)-dependent manner. Prototypic members of this family are Ca(2+)-binding domains of calpain. Our recent finding that grancalcin interacts with L-plastin, a protein known to have actin bundling activity, suggests that grancalcin may play a role in regulation of adherence and migration of neutrophils. The structure of human grancalcin has been determined at 1.9 A resolution in the absence of calcium (R-factor of 0.212 and R-free of 0.249) and at 2. 5 A resolution in the presence of calcium (R-factor of 0.226 and R-free of 0.281). The molecule is predominantly alpha-helical: it contains eight alpha-helices and only two short stretches of two-stranded beta-sheets between the loops of paired EF-hands. Grancalcin forms dimers through the association of the unpaired EF5 hands in a manner similar to that observed in calpain, confirming this mode of association as a paradigm for the PEF family. Only one Ca(2+) was found per dimer under crystallization conditions that included CaCl(2). This cation binds to EF3 in one molecule, while this site in the second molecule of the dimer is unoccupied. This unoccupied site shows higher mobility. The structure determined in the presence of calcium, although does not represent a fully Ca(2+)-loaded form, suggests that calcium induces rather small conformational rearrangements. Comparison with calpain suggests further that the relatively small magnitude of conformational changes invoked by calcium alone may be a characteristic feature of the PEF family. Moreover, the largest differences are localized to the EF1, thus supporting the notion that calcium signaling occurs through this portion of the molecule and that it may involve the N-terminal Gly/Pro rich segment. Electrostatic potential distribution shows significant differences between grancalcin and calpain domain VI demonstrating their distinct character.
DiseaseDisease
Known diseases associated with this structure: Cone dystrophy-3 OMIM:[600364], Hemolytic anemia due to gamma-glutamylcysteine synthetase deficiency OMIM:[606857]
About this StructureAbout this Structure
1F4O is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of human grancalcin, a member of the penta-EF-hand protein family., Jia J, Han Q, Borregaard N, Lollike K, Cygler M, J Mol Biol. 2000 Jul 28;300(5):1271-81. PMID:10903868
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