1f1w: Difference between revisions
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[[Image:1f1w.gif|left|200px]] | [[Image:1f1w.gif|left|200px]] | ||
'''SRC SH2 THREF1TRP MUTANT COMPLEXED WITH THE PHOSPHOPEPTIDE S(PTR)VNVQN''' | {{Structure | ||
|PDB= 1f1w |SIZE=350|CAPTION= <scene name='initialview01'>1f1w</scene>, resolution 2.10Å | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/Transferase Transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.10.1 and 2.7.10.2 2.7.10.1 and 2.7.10.2] | |||
|GENE= | |||
}} | |||
'''SRC SH2 THREF1TRP MUTANT COMPLEXED WITH THE PHOSPHOPEPTIDE S(PTR)VNVQN''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1F1W is a [ | 1F1W is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F1W OCA]. | ||
==Reference== | ==Reference== | ||
Structural basis for specificity switching of the Src SH2 domain., Kimber MS, Nachman J, Cunningham AM, Gish GD, Pawson T, Pai EF, Mol Cell. 2000 Jun;5(6):1043-9. PMID:[http:// | Structural basis for specificity switching of the Src SH2 domain., Kimber MS, Nachman J, Cunningham AM, Gish GD, Pawson T, Pai EF, Mol Cell. 2000 Jun;5(6):1043-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10911998 10911998] | ||
[[Category: Gallus gallus]] | [[Category: Gallus gallus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: src]] | [[Category: src]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:03:13 2008'' | ||
Revision as of 12:03, 20 March 2008
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| , resolution 2.10Å | |||||||
|---|---|---|---|---|---|---|---|
| Activity: | Transferase, with EC number and 2.7.10.2 2.7.10.1 and 2.7.10.2 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
SRC SH2 THREF1TRP MUTANT COMPLEXED WITH THE PHOSPHOPEPTIDE S(PTR)VNVQN
OverviewOverview
The Src SH2 domain binds pYEEI-containing phosphopeptides in an extended conformation with a hydrophobic pocket, which includes ThrEF1, binding Ile(pY +3). Mutating ThrEF1 to tryptophan switches specificity to an Asn(pY +2) requirement, yielding a biological mimic of the Grb2 SH2 domain. Here we show that the Src ThrEF1Trp SH2 domain mutant binds pYVNV phosphopeptides in a beta turn conformation, which, despite differing conformations of the interacting tryptophan, closely resembles the native Grb2/pYVNV cognate peptide binding mode. The ThrEF1Trp substitution therefore switches specificity by physically occluding the pTyr +3 binding pocket and by providing additional interaction surface area for Asn(pY +2). This demonstrates structurally how novel SH2 domain specificities may rapidly evolve through single amino acid substitutions and suggests how new signaling pathways may develop.
About this StructureAbout this Structure
1F1W is a Single protein structure of sequence from Gallus gallus. Full crystallographic information is available from OCA.
ReferenceReference
Structural basis for specificity switching of the Src SH2 domain., Kimber MS, Nachman J, Cunningham AM, Gish GD, Pawson T, Pai EF, Mol Cell. 2000 Jun;5(6):1043-9. PMID:10911998
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