1h9h: Difference between revisions

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-[[Image:1h9h.png|left|200px]]
+==COMPLEX OF EETI-II WITH PORCINE TRYPSIN==
+<StructureSection load='1h9h' size='340' side='right' caption='[[1h9h]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1h9h]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H9H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1H9H FirstGlance]. <br>
+</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene><br>
+<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1aks|1aks]], [[1an1|1an1]], [[1avw|1avw]], [[1avx|1avx]], [[1c9p|1c9p]], [[1d3o|1d3o]], [[1df2|1df2]], [[1eja|1eja]], [[1ept|1ept]], [[1ewu|1ewu]], [[1fmg|1fmg]], [[1fn6|1fn6]], [[1fni|1fni]], [[1ldt|1ldt]], [[1lt2|1lt2]], [[1mct|1mct]], [[1qqu|1qqu]], [[1r0t|1r0t]], [[1s5s|1s5s]], [[1s6f|1s6f]], [[1s6h|1s6h]], [[1s81|1s81]], [[1s82|1s82]], [[1s83|1s83]], [[1s84|1s84]], [[1s85|1s85]], [[1tfx|1tfx]]</td></tr>
+<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] </span></td></tr>
+<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1h9h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1h9h OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1h9h RCSB], [http://www.ebi.ac.uk/pdbsum/1h9h PDBsum]</span></td></tr>
+<table>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/h9/1h9h_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The Ecballium elaterium trypsin inhibitor II (EETI-II) belongs to the family of squash inhibitors and is one of the strongest inhibitors known for trypsin. The eight independent molecules of EETI-II in the crystal structure reported here provide a good opportunity to test the hypothesis that this small cystine-knot protein (knottin) is sufficiently rigid to be used as a molecular scaffold for protein-engineering purposes. To extend this test, the structures of two complexes of EETI-II with trypsin have also been determined, one carrying a four-amino-acid mutation of EETI-II. The remarkable similarity of these structures confirms the rigidity of the molecular framework and hence its suitability as a molecular scaffold.
-{{STRUCTURE_1h9h|  PDB=1h9h  |  SCENE=  }}
+Structure of Ecballium elaterium trypsin inhibitor II (EETI-II): a rigid molecular scaffold.,Kratzner R, Debreczeni JE, Pape T, Schneider TR, Wentzel A, Kolmar H, Sheldrick GM, Uson I Acta Crystallogr D Biol Crystallogr. 2005 Sep;61(Pt 9):1255-62. Epub 2005, Aug 16. PMID:16131759<ref>PMID:16131759</ref>
-===COMPLEX OF EETI-II WITH PORCINE TRYPSIN===
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-{{ABSTRACT_PUBMED_16131759}}
-==About this Structure==
-[[1h9h]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H9H OCA].
 ==See Also==
 *[[Trypsin|Trypsin]]
+== References ==
-==Reference==
+<references/>
-<ref group="xtra">PMID:016131759</ref><ref group="xtra">PMID:010409654</ref><ref group="xtra">PMID:010441159</ref><ref group="xtra">PMID:002622910</ref><references group="xtra"/>
+__TOC__
+</StructureSection>
 [[Category: Sus scrofa]]
 [[Category: Trypsin]]