1ezm: Difference between revisions

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Revision as of 12:02, 20 March 2008

File:1ezm.gif

, resolution 1.5Å

Ligands:

and

Activity:

Pseudolysin, with EC number 3.4.24.26

Coordinates:

save as pdb, mmCIF, xml

THREE-DIMENSIONAL STRUCTURE OF THE ELASTASE OF PSEUDOMONAS AERUGINOSA AT 1.5 ANGSTROMS RESOLUTION

OverviewOverview

Pseudomonas aeruginosa elastase (PAE) is a zinc metalloprotease with 301 amino acids. We have crystallized and solved the three-dimensional structure of PAE, using data to 1.5-A resolution, and have refined the native molecular structure to R = 0.188. The overall tertiary structure of the PAE molecule is similar to that of thermolysin, with which it shares 28% amino acid sequence identity. Nearly all of the active site residues that might potentially interact with substrates are identical in the two proteins. However, the active site cleft is significantly more "open" in PAE than in thermolysin.

About this StructureAbout this Structure

1EZM is a Single protein structure of sequence from [1]. Full crystallographic information is available from OCA.

ReferenceReference

Three-dimensional structure of the elastase of Pseudomonas aeruginosa at 1.5-A resolution., Thayer MM, Flaherty KM, McKay DB, J Biol Chem. 1991 Feb 15;266(5):2864-71. PMID:1899664

Page seeded by OCA on Thu Mar 20 11:02:22 2008

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OCA

@@ Line 1: / Line 1: @@
-[[Image:1ezm.gif|left|200px]]<br /><applet load="1ezm" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1ezm.gif|left|200px]]
-caption="1ezm, resolution 1.5&Aring;" />
-'''THREE-DIMENSIONAL STRUCTURE OF THE ELASTASE OF PSEUDOMONAS AERUGINOSA AT 1.5 ANGSTROMS RESOLUTION'''<br />
+ {{Structure
+|PDB= 1ezm |SIZE=350|CAPTION= <scene name='initialview01'>1ezm</scene>, resolution 1.5&Aring;
+|SITE=
+|LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> and <scene name='pdbligand=CA:CALCIUM ION'>CA</scene>
+|ACTIVITY= [http://en.wikipedia.org/wiki/Pseudolysin Pseudolysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.26 3.4.24.26]
+|GENE=
+}}
+'''THREE-DIMENSIONAL STRUCTURE OF THE ELASTASE OF PSEUDOMONAS AERUGINOSA AT 1.5 ANGSTROMS RESOLUTION'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-EZM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=ZN:'>ZN</scene> and <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Pseudolysin Pseudolysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.26 3.4.24.26] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EZM OCA].
+EZM is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EZM OCA].
 ==Reference==
-Three-dimensional structure of the elastase of Pseudomonas aeruginosa at 1.5-A resolution., Thayer MM, Flaherty KM, McKay DB, J Biol Chem. 1991 Feb 15;266(5):2864-71. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=1899664 1899664]
+Three-dimensional structure of the elastase of Pseudomonas aeruginosa at 1.5-A resolution., Thayer MM, Flaherty KM, McKay DB, J Biol Chem. 1991 Feb 15;266(5):2864-71. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/1899664 1899664]
 [[Category: Pseudolysin]]
 [[Category: Single protein]]
@@ Line 20: / Line 29: @@
 [[Category: hydrolase]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:33:14 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:02:22 2008''