1etk: Difference between revisions
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[[Image:1etk.gif|left|200px]] | [[Image:1etk.gif|left|200px]] | ||
'''THE CRYSTAL STRUCTURE OF E. COLI FIS MUTANT Q68A''' | {{Structure | ||
|PDB= 1etk |SIZE=350|CAPTION= <scene name='initialview01'>1etk</scene>, resolution 2.1Å | |||
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|LIGAND= | |||
|ACTIVITY= | |||
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'''THE CRYSTAL STRUCTURE OF E. COLI FIS MUTANT Q68A''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1ETK is a [ | 1ETK is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ETK OCA]. | ||
==Reference== | ==Reference== | ||
Structural analysis of the transcriptional activation on Fis: crystal structures of six Fis mutants with different activation properties., Cheng YS, Yang WZ, Johnson RC, Yuan HS, J Mol Biol. 2000 Oct 6;302(5):1139-51. PMID:[http:// | Structural analysis of the transcriptional activation on Fis: crystal structures of six Fis mutants with different activation properties., Cheng YS, Yang WZ, Johnson RC, Yuan HS, J Mol Biol. 2000 Oct 6;302(5):1139-51. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11183780 11183780] | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: transcriptional activation region]] | [[Category: transcriptional activation region]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:00:05 2008'' | ||
Revision as of 12:00, 20 March 2008
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THE CRYSTAL STRUCTURE OF E. COLI FIS MUTANT Q68A
OverviewOverview
The Fis protein regulates gene expression in Escherichia coli by activating or repressing transcription of a variety of genes. Fis can activate transcription when bound to DNA upstream of the RNA-polymerase-binding site, such as in the rrnB P1 promoter, or when bound to a site overlapping the -35 RNA polymerase binding site, such as in the proP P2 promoter. It has been suggested that transcriptional activation in both promoters results from interactions between specific amino acids within a turn connecting the B and C helices (the BC turn) in Fis and the C-terminal domain of the alpha-subunit of RNA polymerase (alphaCTD of RNAP). Here, crystal structures of six Fis BC turn mutants with different transcriptional activation properties, Q68A, R71Y, R71L, G72A, G72D and Q74A, were determined at 1.9 to 2.8 A resolution. Two of these mutants, R71Y and R71L, crystallized in unit cells which are different from that of wild-type Fis, and the structure of R71L offers the most complete Fis model to date in that the extended structure of the N-terminal region is revealed. The BC turn in all of these mutant structures remains in a nearly identical gamma gamma beta-turn conformation as present in wild-type Fis. Analyses of the molecular surfaces of the transactivation region of the mutants suggest that several residues in or near the BC turn, including Gln68, Arg71, Gly72 and Gln74, form a ridge that could contact the alphaCTD of RNAP on one side. The structures and biochemical properties of the mutants suggest that Arg71 is the most critical residue for contacting RNAP within this ridge and that the glycine at position 72 helps to stabilize the structure.
About this StructureAbout this Structure
1ETK is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
ReferenceReference
Structural analysis of the transcriptional activation on Fis: crystal structures of six Fis mutants with different activation properties., Cheng YS, Yang WZ, Johnson RC, Yuan HS, J Mol Biol. 2000 Oct 6;302(5):1139-51. PMID:11183780
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