1esg: Difference between revisions
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[[Image:1esg.gif|left|200px]] | [[Image:1esg.gif|left|200px]] | ||
'''RESTRICTION ENDONUCLEASE BAMHI BOUND TO A NON-SPECIFIC DNA.''' | {{Structure | ||
|PDB= 1esg |SIZE=350|CAPTION= <scene name='initialview01'>1esg</scene>, resolution 1.90Å | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] | |||
|GENE= | |||
}} | |||
'''RESTRICTION ENDONUCLEASE BAMHI BOUND TO A NON-SPECIFIC DNA.''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1ESG is a [ | 1ESG is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_amyloliquefaciens Bacillus amyloliquefaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ESG OCA]. | ||
==Reference== | ==Reference== | ||
Structure of BamHI bound to nonspecific DNA: a model for DNA sliding., Viadiu H, Aggarwal AK, Mol Cell. 2000 May;5(5):889-95. PMID:[http:// | Structure of BamHI bound to nonspecific DNA: a model for DNA sliding., Viadiu H, Aggarwal AK, Mol Cell. 2000 May;5(5):889-95. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10882125 10882125] | ||
[[Category: Bacillus amyloliquefaciens]] | [[Category: Bacillus amyloliquefaciens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: non-specific dna-protein complex.]] | [[Category: non-specific dna-protein complex.]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:59:42 2008'' | ||
Revision as of 11:59, 20 March 2008
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| , resolution 1.90Å | |||||||
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| Activity: | Type II site-specific deoxyribonuclease, with EC number 3.1.21.4 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
RESTRICTION ENDONUCLEASE BAMHI BOUND TO A NON-SPECIFIC DNA.
OverviewOverview
The central problem faced by DNA binding proteins is how to select the correct DNA sequence from the sea of nonspecific sequences in a cell. The problem is particularly acute for bacterial restriction enzymes because cleavage at an incorrect DNA site could be lethal. To understand the basis of this selectivity, we report here the crystal structure of endonuclease BamHI bound to noncognate DNA. We show that, despite only a single base pair change in the recognition sequence, the enzyme adopts an open configuration that is on the pathway between free and specifically bound forms of the enzyme. Surprisingly, the DNA drops out of the binding cleft with a total loss of base-specific and backbone contacts. Taken together, the structure provides a remarkable snapshot of an enzyme poised for linear diffusion (rather than cleavage) along the DNA.
About this StructureAbout this Structure
1ESG is a Single protein structure of sequence from Bacillus amyloliquefaciens. Full crystallographic information is available from OCA.
ReferenceReference
Structure of BamHI bound to nonspecific DNA: a model for DNA sliding., Viadiu H, Aggarwal AK, Mol Cell. 2000 May;5(5):889-95. PMID:10882125
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