1esc: Difference between revisions

Revision as of 11:59, 20 March 2008

, resolution 2.1Å

Coordinates:

THE MOLECULAR MECHANISM OF ENANTIORECOGNITION BY ESTERASES

OverviewOverview

The crystal structure of a novel esterase from Streptomyces scabies, a causal agent of the potato scab disease, was solved at 2.1 A resolution. The tertiary fold of the enzyme is substantially different from that of the alpha/beta hydrolase family and unique among all known hydrolases. The active site contains a dyad of Ser 14 and His 283, closely resembling two of the three components of typical Ser-His-Asp(Glu) triads from other serine hydrolases. Proper orientation of the active site imidazol is maintained by a hydrogen bond between the N delta-H group and a main chain oxygen. Thus, the enzyme constitutes the first known natural variation of the chymotrypsin-like triad in which a carboxylic acid is replaced by a neutral hydrogen-bond acceptor.

About this StructureAbout this Structure

1ESC is a Single protein structure of sequence from Streptomyces scabiei. Full crystallographic information is available from OCA.

ReferenceReference

A novel variant of the catalytic triad in the Streptomyces scabies esterase., Wei Y, Schottel JL, Derewenda U, Swenson L, Patkar S, Derewenda ZS, Nat Struct Biol. 1995 Mar;2(3):218-23. PMID:7773790

Page seeded by OCA on Thu Mar 20 10:59:39 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1esc.gif|left|200px]]<br /><applet load="1esc" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1esc.gif|left|200px]]
-caption="1esc, resolution 2.1&Aring;" />
-'''THE MOLECULAR MECHANISM OF ENANTIORECOGNITION BY ESTERASES'''<br />
+ {{Structure
+|PDB= 1esc |SIZE=350|CAPTION= <scene name='initialview01'>1esc</scene>, resolution 2.1&Aring;
+|SITE=
+|LIGAND=
+|ACTIVITY=
+|GENE=
+}}
+'''THE MOLECULAR MECHANISM OF ENANTIORECOGNITION BY ESTERASES'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-ESC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_scabiei Streptomyces scabiei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ESC OCA].
+ESC is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_scabiei Streptomyces scabiei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ESC OCA].
 ==Reference==
-A novel variant of the catalytic triad in the Streptomyces scabies esterase., Wei Y, Schottel JL, Derewenda U, Swenson L, Patkar S, Derewenda ZS, Nat Struct Biol. 1995 Mar;2(3):218-23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7773790 7773790]
+A novel variant of the catalytic triad in the Streptomyces scabies esterase., Wei Y, Schottel JL, Derewenda U, Swenson L, Patkar S, Derewenda ZS, Nat Struct Biol. 1995 Mar;2(3):218-23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7773790 7773790]
 [[Category: Single protein]]
 [[Category: Streptomyces scabiei]]
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 [[Category: hydrolase (serine esterase)]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:30:57 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:59:39 2008''