1erx: Difference between revisions

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Revision as of 11:59, 20 March 2008

File:1erx.gif

, resolution 1.4Å

Ligands:

, and

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CRYSTAL STRUCTURE OF NITROPHORIN 4 COMPLEXED WITH NO

OverviewOverview

The nitrophorins comprise an unusual family of proteins that use ferric (Fe(III)) heme to transport highly reactive nitric oxide (NO) from the salivary gland of a blood sucking bug to the victim, resulting in vasodilation and reduced blood coagulation. We have determined structures of nitrophorin 4 in complexes with H2O, cyanide and nitric oxide. These structures reveal a remarkable feature: the nitrophorins have a broadly open distal pocket in the absence of NO, but upon NO binding, three or more water molecules are expelled and two loops fold into the distal pocket, resulting in the packing of hydrophobic groups around the NO molecule and increased distortion of the heme. In this way, the protein apparently forms a 'hydrophobic trap' for the NO molecule. The structures are very accurate, ranging between 1.6 and 1.4 A resolutions.

About this StructureAbout this Structure

1ERX is a Single protein structure of sequence from Rhodnius prolixus. Full crystallographic information is available from OCA.

ReferenceReference

Nitric oxide binding to nitrophorin 4 induces complete distal pocket burial., Weichsel A, Andersen JF, Roberts SA, Montfort WR, Nat Struct Biol. 2000 Jul;7(7):551-4. PMID:10876239

Page seeded by OCA on Thu Mar 20 10:59:29 2008

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OCA

@@ Line 1: / Line 1: @@
-[[Image:1erx.gif|left|200px]]<br /><applet load="1erx" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1erx.gif|left|200px]]
-caption="1erx, resolution 1.4&Aring;" />
-'''CRYSTAL STRUCTURE OF NITROPHORIN 4 COMPLEXED WITH NO'''<br />
+ {{Structure
+|PDB= 1erx |SIZE=350|CAPTION= <scene name='initialview01'>1erx</scene>, resolution 1.4&Aring;
+|SITE=
+|LIGAND= <scene name='pdbligand=HEV:5,8-DIMETHYL-1,2,3,4-TETRAVINYLPORPHINE-6,7-DIPROPIONIC+ACID+FERROUS+COMPLEX'>HEV</scene>, <scene name='pdbligand=NO:NITROGEN+OXIDE'>NO</scene> and <scene name='pdbligand=CIT:CITRIC ACID'>CIT</scene>
+|ACTIVITY=
+|GENE=
+}}
+'''CRYSTAL STRUCTURE OF NITROPHORIN 4 COMPLEXED WITH NO'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-ERX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rhodnius_prolixus Rhodnius prolixus] with <scene name='pdbligand=HEV:'>HEV</scene>, <scene name='pdbligand=NO:'>NO</scene> and <scene name='pdbligand=CIT:'>CIT</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ERX OCA].
+ERX is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rhodnius_prolixus Rhodnius prolixus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ERX OCA].
 ==Reference==
-Nitric oxide binding to nitrophorin 4 induces complete distal pocket burial., Weichsel A, Andersen JF, Roberts SA, Montfort WR, Nat Struct Biol. 2000 Jul;7(7):551-4. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10876239 10876239]
+Nitric oxide binding to nitrophorin 4 induces complete distal pocket burial., Weichsel A, Andersen JF, Roberts SA, Montfort WR, Nat Struct Biol. 2000 Jul;7(7):551-4. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10876239 10876239]
 [[Category: Rhodnius prolixus]]
 [[Category: Single protein]]
@@ Line 24: / Line 33: @@
 [[Category: nitric oxide complex]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:30:51 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:59:29 2008''