1iaw: Difference between revisions

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[[Image:1iaw.png|left|200px]]
==CRYSTAL STRUCTURE OF NAEI COMPLEXED WITH 17MER DNA==
<StructureSection load='1iaw' size='340' side='right' caption='[[1iaw]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1iaw]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Lechevalieria_aerocolonigenes Lechevalieria aerocolonigenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IAW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1IAW FirstGlance]. <br>
</td></tr><tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1ev7|1ev7]]</td></tr>
<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">NAE I ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=68170 Lechevalieria aerocolonigenes])</td></tr>
<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] </span></td></tr>
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1iaw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1iaw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1iaw RCSB], [http://www.ebi.ac.uk/pdbsum/1iaw PDBsum]</span></td></tr>
<table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
NaeI, a novel DNA endonuclease, shows topoisomerase and recombinase activities when a Lys residue is substituted for Leu 43. The NaeI-DNA structure demonstrates that each of the two domains of NaeI recognizes one molecule of DNA duplex. DNA recognition induces dramatic rearrangements: narrowing the binding site of the Topo domain 16 A to grip DNA, widening that of the Endo domain 8 A to encircle and bend DNA 45 degrees for cleavage, and completely rebuilding the homodimer interface. The NaeI-DNA structure presents the first example of novel recognition of two copies of one DNA sequence by two different amino acid sequences and two different structural motifs in one polypeptide.


{{STRUCTURE_1iaw|  PDB=1iaw  |  SCENE=  }}
Structure of NaeI-DNA complex reveals dual-mode DNA recognition and complete dimer rearrangement.,Huai Q, Colandene JD, Topal MD, Ke H Nat Struct Biol. 2001 Aug;8(8):665-9. PMID:11473254<ref>PMID:11473254</ref>


===CRYSTAL STRUCTURE OF NAEI COMPLEXED WITH 17MER DNA===
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 
</div>
{{ABSTRACT_PUBMED_11473254}}
 
==About this Structure==
[[1iaw]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Lechevalieria_aerocolonigenes Lechevalieria aerocolonigenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IAW OCA].


==See Also==
==See Also==
*[[Endonuclease|Endonuclease]]
*[[Endonuclease|Endonuclease]]
 
== References ==
==Reference==
<references/>
<ref group="xtra">PMID:011473254</ref><references group="xtra"/>
__TOC__
</StructureSection>
[[Category: Lechevalieria aerocolonigenes]]
[[Category: Lechevalieria aerocolonigenes]]
[[Category: Type II site-specific deoxyribonuclease]]
[[Category: Type II site-specific deoxyribonuclease]]

Revision as of 12:09, 28 September 2014

CRYSTAL STRUCTURE OF NAEI COMPLEXED WITH 17MER DNACRYSTAL STRUCTURE OF NAEI COMPLEXED WITH 17MER DNA

Structural highlights

1iaw is a 6 chain structure with sequence from Lechevalieria aerocolonigenes. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:1ev7
Gene:NAE I (Lechevalieria aerocolonigenes)
Activity:Type II site-specific deoxyribonuclease, with EC number 3.1.21.4
Resources:FirstGlance, OCA, RCSB, PDBsum

Publication Abstract from PubMed

NaeI, a novel DNA endonuclease, shows topoisomerase and recombinase activities when a Lys residue is substituted for Leu 43. The NaeI-DNA structure demonstrates that each of the two domains of NaeI recognizes one molecule of DNA duplex. DNA recognition induces dramatic rearrangements: narrowing the binding site of the Topo domain 16 A to grip DNA, widening that of the Endo domain 8 A to encircle and bend DNA 45 degrees for cleavage, and completely rebuilding the homodimer interface. The NaeI-DNA structure presents the first example of novel recognition of two copies of one DNA sequence by two different amino acid sequences and two different structural motifs in one polypeptide.

Structure of NaeI-DNA complex reveals dual-mode DNA recognition and complete dimer rearrangement.,Huai Q, Colandene JD, Topal MD, Ke H Nat Struct Biol. 2001 Aug;8(8):665-9. PMID:11473254[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

See Also

References

  1. ↑ Huai Q, Colandene JD, Topal MD, Ke H. Structure of NaeI-DNA complex reveals dual-mode DNA recognition and complete dimer rearrangement. Nat Struct Biol. 2001 Aug;8(8):665-9. PMID:11473254 doi:10.1038/90366

1iaw, resolution 2.40Å

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