1ek8: Difference between revisions

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CRYSTAL STRUCTURE OF THE RIBOSOME RECYCLING FACTOR (RRF) FROM ESCHERICHIA COLI

OverviewOverview

We have determined the crystal structure of the Escherichia coli ribosome recycling factor (RRF), which catalyzes the disassembly of the termination complex in protein synthesis. The L-shaped molecule consists of two domains: a triple-stranded antiparallel coiled-coil and an alpha/beta domain. The coil domain has a cylindrical shape and negatively charged surface, which are reminiscent of the anticodon arm of tRNA and domain IV of elongation factor EF-G. We suggest that RRF binds to the ribosomal A-site through its coil domain, which is a tRNA mimic. The relative position of the two domains is changed about an axis along the hydrophobic cleft in the hinge where the alkyl chain of a detergent molecule is bound. The tRNA mimicry and the domain movement observed in RRF provide a structural basis for understanding the role of RRF in protein synthesis.

About this StructureAbout this Structure

1EK8 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure of the ribosome recycling factor from Escherichia coli., Kim KK, Min K, Suh SW, EMBO J. 2000 May 15;19(10):2362-70. PMID:10811627

Page seeded by OCA on Thu Mar 20 10:56:44 2008

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OCA

@@ Line 1: / Line 1: @@
-[[Image:1ek8.gif|left|200px]]<br /><applet load="1ek8" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1ek8.gif|left|200px]]
-caption="1ek8, resolution 2.3&Aring;" />
-'''CRYSTAL STRUCTURE OF THE RIBOSOME RECYCLING FACTOR (RRF) FROM ESCHERICHIA COLI'''<br />
+ {{Structure
+|PDB= 1ek8 |SIZE=350|CAPTION= <scene name='initialview01'>1ek8</scene>, resolution 2.3&Aring;
+|SITE=
+|LIGAND= <scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene> and <scene name='pdbligand=DEM:DECYLOXY-METHANOL'>DEM</scene>
+|ACTIVITY=
+|GENE=
+}}
+'''CRYSTAL STRUCTURE OF THE RIBOSOME RECYCLING FACTOR (RRF) FROM ESCHERICHIA COLI'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-EK8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=HG:'>HG</scene> and <scene name='pdbligand=DEM:'>DEM</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EK8 OCA].
+EK8 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EK8 OCA].
 ==Reference==
-Crystal structure of the ribosome recycling factor from Escherichia coli., Kim KK, Min K, Suh SW, EMBO J. 2000 May 15;19(10):2362-70. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10811627 10811627]
+Crystal structure of the ribosome recycling factor from Escherichia coli., Kim KK, Min K, Suh SW, EMBO J. 2000 May 15;19(10):2362-70. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10811627 10811627]
 [[Category: Escherichia coli]]
 [[Category: Single protein]]
@@ Line 23: / Line 32: @@
 [[Category: translation factor]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:28:36 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:56:44 2008''