1eg2: Difference between revisions

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Revision as of 11:55, 20 March 2008

File:1eg2.gif

, resolution 1.75Å

Ligands:

Activity:

Site-specific DNA-methyltransferase (adenine-specific), with EC number 2.1.1.72

Coordinates:

save as pdb, mmCIF, xml

CRYSTAL STRUCTURE OF RHODOBACTER SPHEROIDES (N6 ADENOSINE) METHYLTRANSFERASE (M.RSRI)

OverviewOverview

DNA methylation is important in cellular, developmental and disease processes, as well as in bacterial restriction-modification systems. Methylation of DNA at the amino groups of cytosine and adenine is a common mode of protection against restriction endonucleases afforded by the bacterial methyltransferases. The first structure of an N:6-adenine methyltransferase belonging to the beta class of bacterial methyltransferases is described here. The structure of M. RSR:I from Rhodobacter sphaeroides, which methylates the second adenine of the GAATTC sequence, was determined to 1.75 A resolution using X-ray crystallography. Like other methyltransferases, the enzyme contains the methylase fold and has well-defined substrate binding pockets. The catalytic core most closely resembles the PVU:II methyltransferase, a cytosine amino methyltransferase of the same beta group. The larger nucleotide binding pocket observed in M. RSR:I is expected because it methylates adenine. However, the most striking difference between the RSR:I methyltransferase and the other bacterial enzymes is the structure of the putative DNA target recognition domain, which is formed in part by two helices on an extended arm of the protein on the face of the enzyme opposite the active site. This observation suggests that a dramatic conformational change or oligomerization may take place during DNA binding and methylation.

About this StructureAbout this Structure

1EG2 is a Single protein structure of sequence from Rhodobacter sphaeroides. Full crystallographic information is available from OCA.

ReferenceReference

Structure of RsrI methyltransferase, a member of the N6-adenine beta class of DNA methyltransferases., Scavetta RD, Thomas CB, Walsh MA, Szegedi S, Joachimiak A, Gumport RI, Churchill ME, Nucleic Acids Res. 2000 Oct 15;28(20):3950-61. PMID:11024175

Page seeded by OCA on Thu Mar 20 10:55:08 2008

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OCA

@@ Line 1: / Line 1: @@
-[[Image:1eg2.gif|left|200px]]<br /><applet load="1eg2" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1eg2.gif|left|200px]]
-caption="1eg2, resolution 1.75&Aring;" />
-'''CRYSTAL STRUCTURE OF RHODOBACTER SPHEROIDES (N6 ADENOSINE) METHYLTRANSFERASE (M.RSRI)'''<br />
+ {{Structure
+|PDB= 1eg2 |SIZE=350|CAPTION= <scene name='initialview01'>1eg2</scene>, resolution 1.75&Aring;
+|SITE=
+|LIGAND= <scene name='pdbligand=MTA:5'-DEOXY-5'-METHYLTHIOADENOSINE'>MTA</scene>
+|ACTIVITY= [http://en.wikipedia.org/wiki/Site-specific_DNA-methyltransferase_(adenine-specific) Site-specific DNA-methyltransferase (adenine-specific)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.72 2.1.1.72]
+|GENE=
+}}
+'''CRYSTAL STRUCTURE OF RHODOBACTER SPHEROIDES (N6 ADENOSINE) METHYLTRANSFERASE (M.RSRI)'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-EG2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rhodobacter_sphaeroides Rhodobacter sphaeroides] with <scene name='pdbligand=MTA:'>MTA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Site-specific_DNA-methyltransferase_(adenine-specific) Site-specific DNA-methyltransferase (adenine-specific)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.72 2.1.1.72] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EG2 OCA].
+EG2 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rhodobacter_sphaeroides Rhodobacter sphaeroides]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EG2 OCA].
 ==Reference==
-Structure of RsrI methyltransferase, a member of the N6-adenine beta class of DNA methyltransferases., Scavetta RD, Thomas CB, Walsh MA, Szegedi S, Joachimiak A, Gumport RI, Churchill ME, Nucleic Acids Res. 2000 Oct 15;28(20):3950-61. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11024175 11024175]
+Structure of RsrI methyltransferase, a member of the N6-adenine beta class of DNA methyltransferases., Scavetta RD, Thomas CB, Walsh MA, Szegedi S, Joachimiak A, Gumport RI, Churchill ME, Nucleic Acids Res. 2000 Oct 15;28(20):3950-61. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11024175 11024175]
 [[Category: Rhodobacter sphaeroides]]
 [[Category: Single protein]]
@@ Line 28: / Line 37: @@
 [[Category: exocyclic amino dna methyltransferase rsri]]
 [[Category: mcsg]]
-[[Category: midwest center for structural genomics]]
+[[Category: midwest center for structural genomic]]
 [[Category: protein structure initiative]]
 [[Category: psi]]
 [[Category: rossmann fold]]
-[[Category: structural genomics]]
+[[Category: structural genomic]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:27:22 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:55:08 2008''