4pz7: Difference between revisions
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pz7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pz7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4pz7 RCSB], [http://www.ebi.ac.uk/pdbsum/4pz7 PDBsum]</span></td></tr> | <tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pz7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pz7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4pz7 RCSB], [http://www.ebi.ac.uk/pdbsum/4pz7 PDBsum]</span></td></tr> | ||
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== Publication Abstract from PubMed == | |||
Interactions between RNA guanylyltransferase (GTase) and the C-terminal domain (CTD) repeats of RNA polymerase II (Pol2) and elongation factor Spt5 are thought to orchestrate cotranscriptional capping of nascent mRNAs. The crystal structure of a fission yeast GTase*Pol2 CTD complex reveals a unique docking site on the nucleotidyl transferase domain for an 8-amino-acid Pol2 CTD segment, S5PPSYSPTS5P, bracketed by two Ser5-PO4 marks. Analysis of GTase mutations that disrupt the Pol2 CTD interface shows that at least one of the two Ser5-PO4-binding sites is required for cell viability and that each site is important for cell growth at 37 degrees C. Fission yeast GTase binds the Spt5 CTD at a separate docking site in the OB-fold domain that captures the Trp4 residue of the Spt5 nonapeptide repeat T(1)PAW(4)NSGSK. A disruptive mutation in the Spt5 CTD-binding site of GTase is synthetically lethal with mutations in the Pol2 CTD-binding site, signifying that the Spt5 and Pol2 CTDs cooperate to recruit capping enzyme in vivo. CTD phosphorylation has opposite effects on the interaction of GTase with Pol2 (Ser5-PO4 is required for binding) versus Spt5 (Thr1-PO4 inhibits binding). We propose that the state of Thr1 phosphorylation comprises a binary "Spt5 CTD code" that is read by capping enzyme independent of and parallel to its response to the state of the Pol2 CTD. | |||
How an mRNA capping enzyme reads distinct RNA polymerase II and Spt5 CTD phosphorylation codes.,Doamekpor SK, Sanchez AM, Schwer B, Shuman S, Lima CD Genes Dev. 2014 Jun 15;28(12):1323-36. doi: 10.1101/gad.242768.114. PMID:24939935<ref>PMID:24939935</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
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== References == | |||
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